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  • 1
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Biochemistry 18 (1979), S. 1634-1637 
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Bingley : Emerald
    Rapid prototyping journal 9 (2003), S. 289-300 
    ISSN: 1355-2546
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Traditional rapid prototyping (RP) uses one nozzle, with a single-diameter, to conduct spraying of a RP part. The process is usually time-consuming and inefficient. To improve the traditional method, this research aims to develop a new approach by planning an adaptive path and applying it to the spraying process of every slice. Each accurately sprayed slice then adds one upon another to form a RP part. The processing includes four steps, which will help to produce the adaptive path file, which can be input into the RP machine to make a prototype.
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 451 (1985), S. 0 
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1423-0127
    Keywords: Oligo DNA probe ; α-Sarcin ; Sarcin domain ; 23S rRNA ; Ribosome-inactivating protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The regional structure of the sarcin domain of 23S rRNA ofEscherichia coli ribosomes was determined by a combinatory approach of oligo DNA probes and the action of α-sarcin. The sarcin domain is protected by a reactive complementary oligo DNA probe against the hydrolytic action of α-sarcin. This protective effect is dependent upon the length and the complementary sequence of oligo DNA probes that react to ribosomes. Under UV irradiation and using of the primer extension, nucleotides that contacted by reactive oligo DNA probes were determined. Nucleotides at the 3′ side of the domain (positions from G2659 to C2676) were targeted by oligo DNA probes that have their sequences to complement the domain, indicating that the 3′ side region was exposed on the surface of ribosomes, whereas nucleotides at the 5′ side of stem and extented to two bases at the loop (positions from C2646 to A2654) were not accessible to any oligo DNA probes, implying that the region could be buried in ribosomes. This study also provided evidence that the conformation of the sarcin domain is subjected to alteration if the exposed 3′ side of domain is targeted by the reactive DNA probe. The importance of the topological arrangement of the sarcin domain that engages in the translocation event during translation is discussed.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Journal of biomedical science 7 (2000), S. 420-428 
    ISSN: 1423-0127
    Keywords: Ribosome-inactivating protein ; Ribosome ; Ribonucleic acid ; Ribonuclease ; α-Sarcin ; RNase T1
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Based on the tertiary structure of the ribosome-inactivating protein α-sarcin, domains that are responsible for hydrolyzing ribosomes and naked RNA have been dissected. In this study, we found that the head-to-tail interaction between the first amino β-strand and the last carboxyl β-strand is not involved in catalyzing the hydrolysis of ribosomes or ribonucleic acids. Instead, a four-strand pleated β-sheet is indispensable for catalyzing both substrates, suggesting that α-sarcin and ribonuclease T1 (RNase T1) share a similar catalytic center. The integrity of an amino β-hairpin and that of the loop L3 in α-sarcin are crucial for recognizing and hydrolyzing ribosomes in vitro and in vivo. However, a mutant protein without the β-hairpin structure, or with a disrupted loop L3, is still capable of digesting ribonucleic acids. The functional involvement of the β-hairpin and the loop L3 in the sarcin stem/loop RNA of ribosomes is demonstrated by a docking model, suggesting that the two structures are in essence naturally designed to distinguish ribosome-inactivating proteins from RNase T1 to inactivate ribosomes.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Journal of intelligent manufacturing 5 (1994), S. 411-430 
    ISSN: 1572-8145
    Keywords: Process planning ; rotational parts ; case-based reasoning ; feature-based representation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: This paper introduces a case-based process planning system PROCASE which generates new process routines through learning from existing process routines. In contrast to traditional rule-based systems, the process planning knowledge of the PROCASE is represented in terms of cases instead of production rules. The planning basically comprises case retrieving and case adaptation rather than chaining applicable rules together to form process plans. The advantages are, first, the system is cheaper to build as it saves the expense of knowledge acquisition. Second, the system is able to advance its knowledge automatically through planning practice. Third, it is robust, because the reasoning is not based on pattern matching but similarity comparison. PROCASE has three modules: the retriever, the adapter and the simulator. It is supported by a feature-based representation scheme which naturally serves as the case indices for case retrieving and adaptation. The retriever uses a similarity metric to retrieve an old case which is the most similar case, among all old ones, to the new case. The adapter is then activated to adapt the process plan of the retrieved case to fit the needs for the new case. The simulator is used to verify the feasibility of the adapted plan. PROCASE is implemented on a Silicon Graphics IRIS workstation using C++ . An example is given to demonstrate how the process routine is generated by the system proposed by the authors.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The effect of groups of proteins from rat liver ribosomes on the Escherichia coli stringent factor-catalyzed synthesis of (p)ppGpp was tested. Most groups were capable of supporting (p)ppGpp synthesis; the exceptions were A40, B140, B240 and B160 which contain proteins which are relatively less basic than those in the active groups. The capacity of 30 individual rat liver ribosomal proteins to activate stringent factor was assessed; most sustained the synthesis of (p)ppGpp. Proteins S12, S21, L12, P1, and P2 (which are acidic or relatively acid) had no activity; proteins S6, S8, and L3 were the most active: the others had moderate activity.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 144 (1976), S. 1-9 
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A specially designed apparatus and conditions are described for the rapid analysis of ribosomal proteins by two-dimensional gel electrophoresis on a micro scale. The resolution of proteins in electropherograms is comparable to that obtained with other systems, but because of miniaturization, only 0.5 to 1 μg of each protein is required, and the entire procedure, including electrophoresis in both dimensions, and staining and destaining can be completed in 6 to 7 hours.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    The international journal of advanced manufacturing technology 12 (1996), S. 21-36 
    ISSN: 1433-3015
    Keywords: Case-based reasoning ; Operation sequencing ; Process planning
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract This paper addresses the methodology development for achieving one of the important functions in automated process planning: automated determination of sequence of machining operations for prismatic parts containing various types of form features. A framework of using the case-based reasoning approach to support learning capability in operation sequencing is first proposed. The methodology to accomplish various issues in case-based operation sequencing is then discussed. In particular, the following issues are considered: 1. Representation of operation sequencing cases. 2. Indexing and retrieval of operation sequencing cases. 3. Adaptation of operation sequencing cases. Finally, the implementation issues are addressed and the learning functions of the intelligent operation sequencing system are demonstrated.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    The international journal of advanced manufacturing technology 14 (1998), S. 824-834 
    ISSN: 1433-3015
    Keywords: CAD/CAM ; Reverse engineering
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract This research aims to establish a methodology for error analysis, providing a norm to analyse the margin of error between a prototype and its final product during reverse engineering. It is anticipated that the information derived from such an error analysis is advantageous either for refinement of the product or for modification of a later CAD model. In error analysis, the priority is to establish a universal comparison-standard for the prototype and the final product. In other words, this research calls for a coordinate transformation matrix which transforms the coordinate of measured point-data of a prototype to another coordinate system consistent with that of the final product. The main issues in this research include: 1. Process point-data beforehand and construct a B-spline surface based on these data. 2. Regard statistics as a reference to determine the number of exam-points required for error analysis. 3. Use a simplex method along with a least-squares method to acquire three translation and three rotation parameters for coordinate transformation. 4. Conduct error analysis and display margins of error. In addition to fundamental theory and algorithmic discussion, this research proves the viability of the proposed methodology by examples.
    Type of Medium: Electronic Resource
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