ISSN:
1399-0047
Source:
Crystallography Journals Online : IUCR Backfile Archive 1948-2001
Topics:
Chemistry and Pharmacology
,
Geosciences
,
Physics
Notes:
Uracil-DNA glycosylase (UDG) is a DNA-repair enzyme involved in the removal of uracil from DNA. The Atlantic cod UDG (cUDG) possesses typical cold-adaptation features, with higher catalytic efficiency and lower thermal stability than the mammalian counterparts. cUDG has been crystallized by the vapour-diffusion method using sodium citrate as the precipitant at pH 7.5. The crystals are monoclinic and belong to space group P21, with unit-cell parameters a = 68.58, b = 67.19, c = 68.64 Å, β = 119.85°. There are two molecules in the asymmetric unit, with a corresponding VM value of 2.71 Å3 Da−1 and a solvent content of 54.7%. Synchrotron diffraction data have been collected to 1.9 Å resolution using cryogenic conditions (120 K).
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1107/S0907444901013427
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