ISSN:
1617-4623
Keywords:
Key wordsPyrococcus abyssi
;
Archaea
;
Orotate phosphoribosyltransferase
;
Uracil auxotrophs
;
pyrE gene
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Uracil auxotrophic mutants of the hyperthermophilic archaeon Pyrococcus abyssi were isolated by screening for resistance to 5-fluoro-orotic acid (5-FOA). Wild-type strains were unable to grow on medium containing 5-FOA, whereas mutants grew normally. Enzymatic assays of extracts from wild-type P. abyssi and from pyrimidine auxotrophs demonstrated that the mutants are deficient in orotate phosphoribosyltransferase (PyrE) and/or orotidine-5′-monophosphate decarboxylase (PyrF) activity. The pyrE gene of wild-type P. abyssi and one of its mutant derivatives were cloned and sequenced. This pyrE gene could serve as selectable marker for the development of gene manipulation systems in archaeal hyperthermophiles.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s004380051096
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