ISSN:
1420-9071
Keywords:
Key words. Rat β-globin genes; transcription regulation; MEL cells; REL cells; induction of erythropoiesis.
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract. We show that the rat adult βb miny-globin gene is transcriptionally active. The −100-bp promoter region contains control elements that are important for the induction of transcription in rat erythroleukemia (REL) cells. By using DNaseI footprinting and gel mobility shift assays, we have shown that the CCAAT box, a regulatory element from an analyzed promoter region, binds NF-Y and GATA-1 transcription factors. Another regulatory element from this region, βDRE, binds erythroid-specific and ubiquitous factors from REL cells. Although both the CCAAT box region and βDRE element bind the same protein factors before and after induction of REL cells, we show by South-Western blot analysis that the concentrations of 150-kDa, 70-kDa and 60-kDa factors binding to the βDRE are increased in DMSO-induced REL cells.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/PL00000617
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