ALBERT — All Library Books, journals and Electronic Records Telegrafenberg

Hits per page

hits 1 - 3 | 3 hits

Sorting

Electronic Resource

Development of rat dorsal root ganglion neurones (1974)

Lawson, Sarah N. ; Caddy, K. W. T. ; Biscoe, T. J.

Springer

Cell & tissue research 153 (1974), S. 399-413

add to mindlist on the mindlist

Details

ISSN: 1432-0878

Keywords: Spinal ganglia (Rat) ; Cell division ; Autoradiography ; Neurone morphogenesis ; Light and electron microscopy

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Medicine

Notes: Summary Pulse labelling with tritiated thymidine was used to determine the time of the final division of the neuroblasts which subsequently form rat lumbar dorsal root ganglion neurones. The final division occurred during a 4 day period, the maximum frequency being on day 12 of gestation. Separation of the ganglion cells into large light neurones and small dark neurones showed that the large light neurones were formed earlier than the small dark neurones. In both cases the final divisions occurred over a period of 3–4 days, but the peak rate of formation of large neurones was on day 12, and that of the small neurones was on day 13. Low power electron micrographs were used to measure mean cell diameter throughout development from day 11 of gestation until a postnatal age of 225 days. A marked increase in cell diameter occurred on day 15–15.5, about 3 days after the final cell divisions of the majority of the cells. The rate of growth increased just before birth, but no increase in mean cell diameter was found between day 21 of gestation and the third day postnatal. The growth was again rapid after this period until a plateau in cell diameter was reached about 33 days after birth.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00229167

Permalink

	Location	Call Number	Expected	Availability

Others were also interested in ...

Paper (German National Licenses)

Fulltext

Electronic Resource

Electrophysiological observations on immature rat nerve cells grown in tissue culture (1973)

Lawson, Sarah N. ; Biscoe, T. J.

New York, NY [u.a.] : Wiley-Blackwell

Journal of Cellular Physiology 82 (1973), S. 285-297

add to mindlist on the mindlist

Details

ISSN: 0021-9541

Keywords: Life and Medical Sciences ; Cell & Developmental Biology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Medicine

Notes: Intracellular measurements were made on immature cultured rat neurons using single or double barrelled microelectrodes; 361 dorsal root ganglion, DRG, cells, 105 spinal cord cells and 12 cerebellar cells were studied. Membrane potentials recorded were in the range -25 mV to -50 mV. A statistically significant increase in membrane potentials with time in culture was found for DRG cells during 32 days in culture. This was not found for spinal cord cells.Voltage-current curves showed a non-linearity in about 50% of DRG and spinal cord cells similar to anomalous rectification. Measurements made in DRG and spinal cord cells at ± 10 nA showed significantly greater average cell input resistance during hyperpolarising pulses than during depolarising pulses.The calculated values of Rm and Cm for DRG and spinal cord cells were similar in magnitude to values given for other cells by other workers. The value for L, the dimensionless electrotonic length, was slightly higher than that calculated for motoneurones by other workers. Differences between these results and those of others working on neurons in vitro are ascribed to the immature nature of the cells studied here.

Additional Material: 7 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/jcp.1040820217

Permalink