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  • 1
    Electronic Resource
    Electronic Resource
    Neutron Reflectance Studies of a Novel Nonionic Surfactant and Molecular Modeling of the Surfactant Vesicles (1995)
    s.l. : American Chemical Society
    Langmuir 11 (1995), S. 3737-3741 
    Details
    ISSN: 1520-5827
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/la00010a025
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  • 2
    Electronic Resource
    Electronic Resource
    Self-incompatibility in Papaver rhoeas: there is no evidence for the involvement of stigmatic ribonuclease activity (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Plant, cell & environment 14 (1991), S. 0 
    Details
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract. Ribonuclease assays have revealed, in contrast to the self-incompatibility (SI) system of Nicotiana alata, there is no detectable ribonuclease activity that correlates with the presence of the functional stigmatic S-gene product in Papaver rhoeas. Thus, we have shown that the inhibition of incompatible pollen tube growth in P. rhoeas is not associated with ribonuclease activity. Furthermore, the finding that pollen from P. rhoeas, unlike that from N. alata, is insensitive to purified bovine pancreatic ribonuclease A at very high concentrations, suggests that the involvement of ribonucleases in the inhibition reaction of the SI response in P. rhoeas is unlikely. In addition, the level of ribonuclease activity in mature stigmas of P. rhoeas is very much lower than that in N. alata and significantly, the level of ribonuclease activity did not rise in conjunction with the developmental expression of SI. Therefore, as a result of these studies, we believe that SI in P. rhoeas does not involve ribonuclease activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3040.1991.tb00952.x
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  • 3
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    The Wool Manufacturing Industries in Hong Kong (1966)
    Canberra : Periodicals Archive Online (PAO)
    Quarterly review of the rural economy. 19:3 (1966:July) 152 
    Details
    ISSN: 0156-7446
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Economics
    URL: http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&res_dat=xri:pao:&rft_dat=xri:pao:article:6017-1966-019-03-000004
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  • 4
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    Wool in Mainland China (1962)
    Canberra : Periodicals Archive Online (PAO)
    Quarterly review of the rural economy. 15:4 (1962:Oct.) 188 
    Details
    ISSN: 0156-7446
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Economics
    URL: http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&res_dat=xri:pao:&rft_dat=xri:pao:article:6017-1962-015-04-000005
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  • 5
    Electronic Resource
    Electronic Resource
    Selected genetics (1979)
    [s.l.] : Nature Publishing Group
    Nature 282 (1979), S. 173-173 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Understanding Genetics. By E.B. Ford. Pp. 201. (Faber and Faber: London, 1979.) £7.25. PROFESSOR FORD tells us in the preface that this book is an attempt to meet the needs of those who, whether they be men of general education or scholars in other fields of learning, wish to know something ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/282173a0
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  • 6
    Electronic Resource
    Electronic Resource
    Genotypic Control of Crossing-Over on the First Chromosome of Drosophila melanogaster (1958)
    [s.l.] : Nature Publishing Group
    Nature 182 (1958), S. 889-890 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The experiment was designed to determine the effect of the autosomal genotype on the recombination between three marker genes on the X-chromo-some, namely, white-eye (w), miniature wing (m) and bar-eye (B) (Fig. 1). Two long-inbred lines of D. melanogaster, Oregon (0) and Samarkand (S), together ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/182889a0
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  • 7
    Electronic Resource
    Electronic Resource
    Molecular analysis of two functional homologues of the S 3 allele of the Papaver rhoeas self-incompatibility gene isolated from different populations (1996)
    Springer
    Plant molecular biology 30 (1996), S. 983-994 
    Details
    ISSN: 1573-5028
    Keywords: self-incompatibility ; Papaver rhoeas ; S gene ; homologous alleles ; recombinant S protein ; pollen inhibition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The S 3 allele of the S gene has been cloned from Papaver rhoeas cv. Shirley. The sequence predicts a hydrophilic protein of 14.0 kDa, showing 55.8% identity with the previously cloned S 1 allele, preceded by an 18 amino acid signal sequence. Expression of the S 3 coding region in Escherichia coli produced a form of the protein, denoted S3e, which specifically inhibited S3 pollen in an in vitro bioassay. The recombinant protein was ca. 0.8 kDa larger than the native stigmatic form, indicating post-translational modifications in planta, as was previously suggested for the S1 protein. In contrast to other S proteins identified to date, S3 protein does not appear to be glycosylated. Of particular significance is the finding that despite exhibiting a high degree of sequence polymorphism, secondary structure predictions indicate that the S1 and S3 proteins may adopt a virtually identical conformation. Sequence analysis also indicates that the P. rhoeas S alleles share some limited homology with the SLG and SRK genes from Brassica oleracea. Previously, cross-classification of different populations of P. rhoeas had revealed a number of functionally identical alleles. Probing of western blots of stigma proteins from plants derived from a wild Spanish population which contained an allele functionally identical to the Shirley S 3 allele with antiserum raised to S3e, revealed a protein (S 3 s) which was indistinguishable in pI and M r from that in the Shirley population. A cDNA encoding S 3 s was isolated, nucleotide sequencing revealing a coding region with 99.4% homology with the Shirley-derived clone at the DNA level, and 100% homology at the amino acid level.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00020809
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  • 8
    Electronic Resource
    Electronic Resource
    Systematic Investigations of the Influence of Molecular Structure on the Transport of Peptides Across Cultured Alveolar Cell Monolayers (2000)
    Springer
    Pharmaceutical research 17 (2000), S. 7-14 
    Details
    ISSN: 1573-904X
    Keywords: transport of peptides ; molecular structure ; molecular size ; cultured alveolar cell monolayers ; alveolar permeability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Purpose. To determine how the structures of peptides influence theiralveolar permeability. Methods. The studies were performed using 14 synthetic ‘model’peptides, labelled with a novel, non-intrusive amino acid fluorophore, andtheir transport studied using rat alveolar cell monolayers cultured onpermeable supports. Results. The passage of the peptides across the epithelial cellmonolayers is shown to be primarily paracellular, with an inverse dependenceon molecular size, and an enhanced flux observed for cationic peptides.The apparent permeability coefficients (P app ) for the peptides(together with those for other organic solutes, taken from the literature) areshown to be well-modelled assuming two populations of ‘pores’ in themonolayers, modelled as cylindrical channels of radii 15 Å and 22nm. The former pores are shown to be numerically equatable withthe monolayer tri-junctional complexes, and the latter are taken asmonolayer defects. Conclusions. The various monolayer P app values correlatewell with the results from in vivo transport experiments, and the conclusion isdrawn that the pulmonary delivery of peptide drugs is perfectlyexploitable.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1007514121527
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  • 9
    Electronic Resource
    Electronic Resource
    Molecular basis of the incompatibility mechanism in Papaver rhoeas L. (1992)
    Springer
    Plant growth regulation 11 (1992), S. 5-12 
    Details
    ISSN: 1573-5087
    Keywords: Papaver ; self-incompatibility ; pollen gene expression ; ribonuclease
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In this study we have begun to dissect the molecular mechanism of the self-incompatibility reaction of Papaver rhoeas. In order to gain an insight into the cellular activities, which lead to the inhibition of pollen tube growth following a self-incompatible response, we have been studying the effects of various metabolic inhibitors on pollen-stigmatic extract interactions in vitro. The results indicate that both transcription and glycosylation are required for the full inhibition of pollen tube growth during an incompatible response in P. rhoeas. The ability of actinomycin D to alleviate an incompatible reaction suggests that during the response pollen gene expression is induced; we have evidence that this is indeed the case and have identified novel proteins produced in the pollen which are associated with the incompatibility response. These findings give a clear indication that de novo transcription of pollen genes which are specific to this response, play an important role in the inhibition of pollen tube growth in this species. This provides a significant step towards the elucidation of the mechanism whereby pollen tube growth is arrested following an incompatible reaction in this species. Ribonuclease assays have revealed that, in contrast to the S-linked glycoprotein of Nicotiana alata, there is no detectable ribonuclease activity that correlates with the presence of the functional stigmatic S-gene product in P. rhoeas.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00024426
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  • 10
    Electronic Resource
    Electronic Resource
    Genetics of genetic conservation. I. Sample size when collecting germplasm (1995)
    Springer
    Euphytica 84 (1995), S. 89-99 
    Details
    ISSN: 1573-5060
    Keywords: genetic conservation ; minimum sample size ; self-fertilising and cross-fertilising species
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A sample of about 172 plants, drawn at random from a population of a target species, is of sufficient size to conserve at a very high probability, all or very nearly all of the polymorphic genes that are segregating in the population, provided that their frequency is not less than 0.05, irrespective of whether the individuals of the species set all of their seed by self-or by cross-fertilisation or a mixture of both. When samples are taken from a number of populations, the size of the sample drawn from each need be no larger than 172 divided by the number of populations visited. It is pointed out that implementation of this conclusion could lead to very considerable saving of resources in both the collection and storage of material in gene banks.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01677945
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