ISSN:
1432-072X
Keywords:
Bacillus licheniformis
;
Teichoic and teichuronic acids
;
Protein export
;
Na+ and gramidicin inhibition
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Teichuronic acid was the major anionic polymer of Bacillus licheniformis NCIB 6346 durign phosphate-limited (P-limited) growth in the chemostat. This polymer was also present in significant quantities when B. licheniformis was grown under carbon-limited (C-limited) or magnesium-limited (Mg-limited) conditions where teichoic acid predominated in the cell wall. However, the cell wall composition was not of significance in protein export and the parameters for the excretion process were found to be environmental. In particular, two types of extracellular proteins were identified: the first type of enzyme, penicillinase, was only weakly catabolite repressed; was maximally synthesized and secreted during P-limited growth; was unaffected by growth in high Na+ media but its production was inhibited by gramicidin. The second type of enzyme, α-amylase, was strongly catabolite repressed and its export was markedly inhibited during P-limited growth or in the presence of Na+ or gramicidin. It is noteworthy that the penicillinase carries a glyceride-cysteine modification at its N-terminus whilst the α-amylase does not.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00415277
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