ISSN:
1432-2242
Keywords:
Lycopersicon esculentum
;
Lycopersicon peruvianum
;
RAPD
;
RFLP
;
Tomato spotted wiltvirus (TSWV)
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The Sw-5 locus confers dominant resistance to tomato spotted wilt virus (TSWV). To map the location and facilitate the identification of markers linked to Sw-5 we developed a pair of near-isogenic lines (NILs) and an F2 Lycopersicon esculentum x L. pennellii population segregating for resistance to TSWV. DNA from the NILs was analyzed using 748 random 10-mer oligonucleotides to discern linked molecular markers using a random amplified polymorphic DNA (RAPD) approach. One random primer (GAGCACGGGA) was found to produce a RAPD band of about 2200 bp that demonstrates linkage to Sw-5. Data from co-segregation of resistance and restriction fragment length polymorphisms (RFLPs) in a F2 interspecific population position Sw-5 between the markers CT71 and CT220 near the telomere of the long arm of chromosome 9.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00221989
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