ISSN:
1573-5176
Keywords:
flow cytometry
;
diatom
;
Phaeodactylum tricornutum
;
cell cycle
;
nocodazole
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The microtubule inhibitor, nocodazole (2.5 mg L-1), can arrest the cell cycle of the pennate diatom Phaeodactylum tricornutum Bohlin at G2 + M phase. Flow cytometric analysis of cells treated with nocodazole suggest that the proportion of cells at G2 + M phase can accumulate to over 95%. Even after a 48-h treatment with nocodazole (2.5 mg L-1), the cells can still exit mitosis, suggesting that the cell-cycle arrest is reversible.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1008039500919
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