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  • 1
    ISSN: 1365-2427
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SUMMARY. 1. The effect of removal of organic manor 〉1000 apparent molecular weight (AMW) upon biofilm processes was determined in three contrasting streams in West Germany and three contrasting rivers in the U.K. This process removed 66–85% of the dissolved organic matter supply.2. Elevations in extra cellular enzyme activity (β-glucosidase, phosphatase and esterase), metabolic heat-output, bacterial density and poly-beta-hydroxy alkanoate (PHA) content (a prokaryote storage product) were noted throughout the study in response to the removal of organic matter. This suggests that there are inhibitory substances present in the dissolved organic matter pool 〉1000 AMW. Evidence is presented to suggest that phenolics play a role in this inhibition.3. Decreases in metabolic heat output, β-glucosidase activity and PHA content were noted at four sites in response to the removal of 〉1000 AMW material.4. The divergent responses of the six river/stream biofilms are indicative of radically differing metabolic/catabolic processes, on a spatial and/or temporal basis, to a major organic supply perturbation.
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Freshwater biology 16 (1986), S. 0 
    ISSN: 1365-2427
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SUMMARY. 1. Colloidal and dissolved organic carbon (CDOC) was monitored in six pristine boreal rivers in Northern Quebec, Canada, using ultrafiltration to obtain apparent molecular weight spectra.2. The concentration and proportional contribution of small (〈1K) molecular weight material (potentially microbially labile) and high (0.7μm to 300 K) molecular weight material was not significantly different between streams of order 2–9.3. The dominant component of CDOC (0.7μm to 300 K fraction) was probably derived in streams other than first order, from surface or sub-surface run-off, whereas a peak concentration in the 〈1 K fraction during August was probably due to autochthonous exudates.4. The two first order streams included in this survey had very low CDOC concentrations. However, further work on twelve other first order streams showed that they had high concentrations and we consider that this is the more typical situation. The finding offered a potential explanation for the otherwise anomalous longitudinal CDOC distribution pattern throughout the study area.
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Freshwater biology 26 (1991), S. 0 
    ISSN: 1365-2427
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SUMMARY. 1. Glycogen, poly-beta-hydroxyalkanoate (PHA) and electron transport system (ETS) activity levels were monitored seasonally in light- and dark-grown biofilms from a North Wales river.2. A spring peak was evident in glycogen, PHA and ETS activity levels per cm2 and per cell, with the exception of PHA per cell, which peaked in the winter.3. There was a seasonal shift in the type of storage product found, perhaps reflecting seasonal changes in carbon sources.4. The light-grown spring biofilm yielded the greatest amount of bio-film storage products per cm2. This should be taken into account during caloric studies of river biofilms as a food source for grazing invertebrates.5. A model is presented of factors influencing storage product deposition in river biofilms. The thickness and cell packing of river biofilms should be noted in future studies. Storage product deposition can occur deep within thick biofilms, irrespective of the river water chemistry.
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Freshwater biology 26 (1991), S. 0 
    ISSN: 1365-2427
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SUMMARY. 1. In laboratory experiments, 9.7–25.7% of dissolved organic carbon (DOC) in groundwater (at concentrations of 18.7–24.8 mg 1-−1) was immobilized after perfusion through 8-cm-deep (22-cm-diameter) cores of stony stream-bed substratum.2. This represented immobilization rates of 7.1–23.5 mg m−2 h−1 or, extrapolated across the year, potential immobilization rates within the stream bed of 62.2–205.9g m−2 yr−1. Actual rates in the entire stream bed were probably higher because perfusion through the experimental cores did not reduce groundwater DOC concentrations to levels measured in the adjacent stream.3. Natural concentrations of dissolved free amino acids (DFAAs) in groundwater were generally unchanged following perfusion through the cores, suggesting the maintenance of a dynamic equilibrium in their concentrations.4. Selective enrichments of amino acids in groundwater (up to 20-fold) were entirely immobilized following perfusion, indicating their rapid retention and flux in this environment. Thus, immobilization of the bulk DOC in stream-bed cores probably did not reflect net reductions in dissolved free, low-molecular-weight material, with higher molecular weight, more ‘refractory’ material being immobilized instead.5. We conclude that groundwater can contribute substantial amounts of DOC, both high and low molecular weight, to a stream ecosystem. The stream bed is the site at which much of this material could be initially immobilized and made available to the stream trophic structure.
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Freshwater biology 22 (1989), S. 0 
    ISSN: 1365-2427
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SUMMARY. 1. Extracellular hydrolytic enzyme activities and cell densities were monitored during undisrupted biofilm formation on pristine surfaces in two contrasting river sites in North Wales: an oligotrophic mountain stream (Nant Waen) and a mildly eutrophic river (River Clywedog).2. Bacterial densities generally increased at both sites over a 33-day monitoring period. Densities in the eutrophic site were approximately 14 times greater than in the mountain stream.3. Using fluorescent substrate analogues, biofilms from Nant Waen produced low, variable xylosidase and β-glucosidase activities. Biofilms from the more eutrophic River Clywedog produced higher xylosidase and β-glucosidase activities and detectable endopeptidase, though these activities also fluctuated during the colonization period.4. Unlike the other activities measured, esterase activities in the River Clywedog were correlated with cell densities (P〈0.05). When extracellular esterase activities per cell were calculated, the oligotrophic biofilm was found to contain about twice as much extracellular esterase activity as the more eutrophic River Clywedog biofilm.
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  • 6
    ISSN: 1365-2427
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: A laboratory stream is described which has simulated steep- and shallow-gradient bottoms whose gradients can be varied. It was found that Polycelis felina, the principal triclad of shallow-gradient streams had a much reduced ability to colonize the simulated steep-gradient bottoms when compared to Crenobia alpina, which is the principal triclad of the steep-gradient bottoms in the field. P. felina is unable to move in the steep-gradient bottoms to any great extent, while C. alpina showed considerable ability to do this. When a reduction in flow was made this did not increase the proportion of P. felina to be found in the steep-gradient regions. The triclads were also tested for possible acclimatory responses and it was found that their responses were more fundamentally based. On the basis of the above evidence it is suggested that the absence of P. felina from the steep-gradient regions of streams in North Wales is due to a behavioural inability to tolerate the current regimes present in the steep-gradient bottoms.
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  • 7
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 45 (1987), S. 0 
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The utilisation of colloidal and dissolved organic carbon (CDOC) by boreal river epilithon was investigated. The technique of microcalorimetry was used to determine the metabolic response of the epilithon in terms of both absolute levels and utilisation of material of apparent molecular weight (AMW) above and below 1000. Short-term perfusions were used to measure absolute levels of metabolic heat output for comparison with other studies, and long-term perfusions were used to study the relative utilisation of different molecular weight fractions. The results indicated low levels of metabolic heat output from boreal river epilithon with a shorter lag period in the utilisation of CDOC than we have found in a temperature river system. Long-term perfusions indicated a considerable increase in metabolic heat output on removal of high-molecular-weight material. We discuss these results in terms of a ‘competitive access model’ whereby slowly metabolised high-molecular-weight compounds saturate adsorption sites on the epilithon surface, resulting in a low metabolic heat output. When these compounds are removed, ‘labile’ low-molecular-weight molecules may more readily adsorb to or diffuse into the epilithon matrix.
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  • 8
    ISSN: 1432-1939
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary An apparatus is described which allows triclads to choose between the fast and slow regions of a stirred tank. In the absence of a stone substratum Crenobia alpina preferred or tolerated higher current speeds than Polycelis felina, this effect was enhanced when a stone substratum was added to the apparatus. These results indicate that the absence of Polycelis felina from the steep gradient regions of streams could be accounted for by its preference for slower micro-currents. Conversely the presence of Crenobia alpina in steep gradient regions could be accounted for by its preference for or tolerance to higher micro-currents.
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  • 9
    ISSN: 1573-5117
    Keywords: river biofilm ; environmental change ; acclimation ; PHA ; extracellular enzymes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Endogenous carbon reserves such as poly-beta-hydroxy alkanoate (PHA) can sustain microbial viability during conditions of nutrient deprivation. Microbial extracellular enzyme activities under one set of environmental conditions might be wholly inappropriate for another, and thus PHA might also serve as an energy source as the biofilm acclimates to a changed environment. In order to test this hypothesis, radical changes in environmental conditions were imposed upon river biofilms by transferring them between three rivers of acid, circum-neutral and alkaline pH. The findings supported the hypothesis; each of the transfers resulted in reduced PHA levels, while the physiology of the biofilm (metabolic activity, population density, phosphatase & glucosidase activities) acclimated to the environmental conditions of the recipient site. The greatest PHA depletion was observed when the magnitude of the imposed change resulted in an inability of phosphatase enzyme to respond to the change. The implicit greater dependence on the reserves of PHA, is similarly consistent with the hypothesis.
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Journal of aquatic ecosystem stress and recovery 2 (1973), S. 329-334 
    ISSN: 1573-5141
    Keywords: biofilm ; activity ; lipid synthesis ; 14C acetate ; calorimetry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A method is described which enables lipid biosynthesis to be determined within intact river biofilms. Significantly different rates of biosynthesis were detected in rivers of differing nutrient availability and during different seasons. Rapid changes in microbial physiology could be detected within 24 hours. The technique appeared to be well suited to investigation of factors affecting lipid biosynthesis within biofilms. Although in contrast, acetate incorporation did not correlate with microcalorimetric total activity measurements over a 12-month period, and so the method did not appear suitable for determining total metabolic activity. However, microbial lipid biosynthesis produces a valuable food resource for the ecosystems higher tropic levels and thus the acetate incorporation technique could prove useful as an indicator of aspects of aquatic ecosystem health.
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