Publication Date:
1992-06-19
Description:
A triple helix is formed upon binding of an oligodeoxynucleotide to the major groove of duplex DNA. A benzo[e]pyridoindole derivative (BePI) strongly stabilized this structure and showed preferential binding to a triplex rather than to a duplex. Energy transfer experiments suggest that BePI intercalates within the triple helix. Sequence-specific inhibition of transcription initiation of a specific gene by Escherichia coli RNA polymerase by a triplex-forming oligodeoxynucleotide is strongly enhanced when the triplex is stabilized by BePI. Upon irradiation with ultraviolet light, BePI induces covalent modifications of the target within the triple helix structure.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Mergny, J L -- Duval-Valentin, G -- Nguyen, C H -- Perrouault, L -- Faucon, B -- Rougee, M -- Montenay-Garestier, T -- Bisagni, E -- Helene, C -- New York, N.Y. -- Science. 1992 Jun 19;256(5064):1681-4.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Laboratoire de Biophysique, Institut National de la Sante et de la Recherche Medicale, Paris, France.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/1609278" target="_blank"〉PubMed〈/a〉
Keywords:
Base Sequence
;
Carbolines/metabolism
;
DNA/genetics/*metabolism
;
DNA-Directed RNA Polymerases/genetics/metabolism
;
Escherichia coli/*genetics
;
Hot Temperature
;
In Vitro Techniques
;
*Ligands
;
Molecular Sequence Data
;
Nucleic Acid Hybridization
;
Transcription, Genetic
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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