ISSN:
1617-4623
Keywords:
Mating type
;
Yarrowia lipolytica
;
Protoplast fusion
;
Sporulation
;
Conjugation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary The mating type gene MA TA of the dimorphic yeast Yarrowia lipolytica was cloned. The strategy used was based on the presumed function of this gene in the induction of sporulation. A diploid strain homozygous for the mating type B was transformed with an integrative gene bank from an A wild-type strain. A sporulating transformant was isolated, which contained a plasmid with an 11.6 kb insert. This sequence was rescued from the chromosomal DNA of the transformant and deletion mapping was performed to localize the MAT insert. The MAT gene conferred both sporulating and non-mating phenotypes on a B/B diploid. A LEU2 sequence targeted to this locus segregated like a mating type-linked gene. The A strain did not contain silent copies of the MAT gene.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00266246
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