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  • 1
    Electronic Resource
    Electronic Resource
    Multiple knockout analysis of genetic robustness in the yeast metabolic network (2006)
    [s.l.] : Nature Publishing Group
    Nature genetics 38 (2006), S. 993-998 
    Details
    ISSN: 1546-1718
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] Genetic robustness characterizes the constancy of the phenotype in face of heritable perturbations. Previous investigations have used comprehensive single and double gene knockouts to study gene essentiality and pairwise gene interactions in the yeast Saccharomyces cerevisiae. Here we conduct an in ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/ng1856
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  • 2
    Electronic Resource
    Electronic Resource
    A genome-wide analysis in Saccharomyces cerevisiae demonstrates the influence of chromatin modifiers on transcription (2007)
    [s.l.] : Nature Publishing Group
    Nature genetics 39 (2007), S. 303-309 
    Details
    ISSN: 1546-1718
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] Chromatin structure is important in transcription regulation. Many factors influencing chromatin structure have been identified, but the transcriptional programs in which they participate are still poorly understood. Chromatin modifiers participate in transcriptional control together with DNA-bound ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/ng1965
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  • 3
    Electronic Resource
    Electronic Resource
    The RAD50 gene of Saccharomyces cerevisiae is not essential for vegetative growth (1986)
    Springer
    Current genetics 10 (1986), S. 487-489 
    Details
    ISSN: 1432-0983
    Keywords: DNA repair ; Gene disruption
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The gene RAD50 was located by the ability of subclones to restore the Rad+ phenotype following transformation of a rad50-1 mutant. Disruption of the gene was achieved by directed integration of a plasmid carrying a fragment internal to RAD50. Haploids with the disrupted gene are viable and do not differ in growth rate or plating efficiency from isogenic rad50-1 or Rad+ strains.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00419878
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  • 4
    Electronic Resource
    Electronic Resource
    Characterization of the role played by the RAD59 gene of Saccharomyces cerevisiae in ectopic recombination (1999)
    Springer
    Current genetics 36 (1999), S. 13-20 
    Details
    ISSN: 1432-0983
    Keywords: Key words Gene conversion ; DNA repair ; Ty elements ; Genomic instability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The RAD52 group of genes in the yeast Saccharomyces cerevisiae controls the repair of DNA damage by a recombinational mechanism. Despite the growing evidence for physical and biochemical interactions between the proteins of this repair group, mutations in individual genes show very different effects on various types of recombination. The RAD59 gene encodes a protein with similarity to Rad52p which plays a role in the repair of damage caused by ionizing radiation. In the present study we have examined the role played by the Rad59 protein in mitotic ectopic recombination and analyzed the genetic interactions with other members of the repair group. We found that Rad59p plays a role in ectopic gene conversion that depends on the presence of Rad52p but is independent of the function of the RecA homologue Rad51p and of the Rad57 protein. The RAD59 gene product also participates in the RAD1-dependent pathway of recombination between direct repeats. We propose that Rad59p may act in a salvage mechanism that operates when the Rad51 filament is not functional.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002940050467
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  • 5
    Electronic Resource
    Electronic Resource
    Donation of information to the unbroken chromosome in double-strand break repair (1993)
    Springer
    Current genetics 23 (1993), S. 414-422 
    Details
    ISSN: 1432-0983
    Keywords: Saccharomyces cerevisiae ; Donation ; Gene conversion ; Double-strand break repair ; Heteroduplex DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have used transformation of yeast with lincarized plasmids to study the transfer of information to the unbroken chromosome during double-strand break repair. Using a strain which carried the wild-type HIS3 allele, and a linearized plasmid which carried a mutant his3 allele, we have obtained His- transformants. In these, double-strand break repair has resulted in precise transfer of genetic information from the plasmid to the chromosome. Such repair events, we suggest, are gene conversions which entail the formation of heteroduplex DNA on the (unbroken) chromosome. If this suggestion is correct, our results reflect the spatial distribution of such heteroduplex DNA. Transfer of information from the plasmid to the chromosome was obtained at a maximal frequency of 1.5% of the repair events, and showed a dependence with distance. Transformation to His- was also obtained with a 2-kbp insertion and with a deletion of 200 bp. The latter results suggest that gene conversion of large heterologies can occur via repair of a heteroduplex DNA intermediate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00312628
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  • 6
    Electronic Resource
    Electronic Resource
    Recombination between divergent sequences leads to cell death in a mismatch-repair-independent manner (2000)
    Springer
    Current genetics 38 (2000), S. 23-32 
    Details
    ISSN: 1432-0983
    Keywords: Key words Gene conversion ; Double-strand breaks ; DNA repair ; Mismatch repair
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Homologous recombination is an important DNA repair mechanism in vegetative cells. During the repair of double-strand breaks, genetic information is transferred between the interacting DNA sequences, thus creating a gene-conversion event. Gene conversion of a functional member of a gene family, which uses an inactive member (such as a pseudogene) as a template, might have deleterious consequences. It is therefore important for the cell to prevent recombination between divergent sequences. We have studied the repair of a double-strand break by recombination in a haploid yeast strain carrying 99% identical alleles located on different chromosomes. The fate of the broken chromosome was followed in the whole cell population without imposing selective constraints. Our results show that all the cells were able to repair the broken chromosome by gene conversion. During the repair, the cells arrest in the cell cycle with a “dumbbell” configuration characteristic of G2/M-arrested cells. Surprisingly, although all the cells repaired the broken chromosome, 60% of them were unable to resume growth and to form colonies after the repair was completed. The low level of cell recovery was due to the 1% divergence between the alleles, but was not dependent on the function of the mismatch-repair system. Cell death, however, could be prevented by the presence of an alternative source of perfect homology located on a different chromosome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002940000124
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  • 7
    Electronic Resource
    Electronic Resource
    Cloning and mapping of CDC40, a Saccharomyces cerevisiae gene with a role in DNA repair (1985)
    Springer
    Current genetics 9 (1985), S. 253-257 
    Details
    ISSN: 1432-0983
    Keywords: Saccharomyces cerevisiae ; CDC40 ; DNA repair ; Cloning ; Mapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The cdc40 mutation has been previously shown to be a heat-sensitive cell-division-cycle mutation. At the restrictive temperature, cdc40 cells arrest at the end of DNA replication, but retain sensitivity to hydroxyurea (Kassir and Simchen 1978). The mutation has also been shown to affect commitment to meiotic recombination and its realization. Here we show that mutant cells are extremely sensitive to Methyl-Methane Sulfonate (MMS) when the treatment is carried out at restrictive temperature. Incubation at 37 °C prior to, or after MMS treatment at 23 °C, does not result in lower survival. It is concluded that the CDC40 gene product has a role in DNA repair, possibly holding together or protecting the DNA during the early stages of repair. The CDC40 gene was cloned on a 2.65 kb DNA fragment. A 2 μ plasmid carrying the gene was integrated and mapped to chromosome IV, between trp4 and ade8, by the method of marker loss. Conventional tetrad analysis has shown cdc40 to map 1.7 cM from trp4.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00419952
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  • 8
    Electronic Resource
    Electronic Resource
    Cloning and integrative deletion of the RAD6 gene of Saccharomyces cerevisiae (1984)
    Springer
    Current genetics 8 (1984), S. 559-566 
    Details
    ISSN: 1432-0983
    Keywords: DNA repair ; Saccharomyces cerevisiae ; Cloning
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Three overlapping plasmids were isolated from a YEp24 library, which restore Rad+ functions to rad6-1 and rad6-3 mutants. Different subclones were made and shown to integrate by homologous recombination at the RAD6 site on chromosome VII, thus verifying the cloned DNA segments to be the RAD6 gene and not a suppressor. The gene resides in a 1.15 kb fragment, which restores Rad+ levels of resistance to U.V., MMS and γ-rays to both rad6-1 and rad6-3 strains. It also restores sporulation ability to rad6-1 diploids. Integrative deletion of the RAD6 gene was shown not to be completely lethal to the yeast. Our results suggest that the RAD6 gene has some cell cycle-specific function(s), probably during late S phase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00395700
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  • 9
    Electronic Resource
    Electronic Resource
    The role ofSaccharomyces cerevisiae Cdc40p in DNA replication and mitotic spindle formation and/or maintenance (1995)
    Springer
    Molecular genetics and genomics 247 (1995), S. 123-136 
    Details
    ISSN: 1617-4623
    Keywords: CDC40 ; DNA replication ; Mitotic spindle assembly ; cyclins ; Saccharomyces cerevisiae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Successful progression through the cell cycle requires the coupling of mitotic spindle formation to DNA replication. In this report we present evidence suggesting that, inSaccharomyces cerevisiae, theCDC40 gene product is required to regulate both DNA replication and mitotic spindle formation. The deduced amino acid sequence ofCDC40 (455 amino acids) contains four copies of a β-transducin-like repeat. Cdc40p is essential only at elevated temperatures, as a complete deletion or a truncated protein (deletion of the C-terminal 217 amino acids in thecdc40-1 allele) results in normal vegetative growth at 23°C, and cell cycle arrest at 36°C. In the mitotic cell cycle Cdc40p is apparently required for at least two steps: (1) for entry into S phase (neither DNA synthesis, nor mitotic spindle formation occurs at 36°C and (2) for completion of S-phase (cdc40::LEU2 cells cannot complete the cell cycle when returned to the permissive temperature in the presence of hydroxyurea). The role of Cdc40p as a regulatory protein linking DNA synthesis, spindle assembly/maintenance, and maturation promoting factor (MPF) activity is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00705642
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  • 10
    Electronic Resource
    Electronic Resource
    Cloning and mapping of the RAD50 gene of Saccharomyces cerevisiae (1984)
    Springer
    Molecular genetics and genomics 193 (1984), S. 525-531 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The RAD50 gene was cloned as a 4.8 kb fragment in the 2 μ derived plasmid pFL1. The gene resides in a 3.9 kb segment that was subcloned into the plasmid YRp7. The cloned gene complements the deficiency caused by the rad50-1 mutation with respect to γ-rays, MMS resistance and UV-induced mitotic recombination. Restoration of the Rad+ phenotype occurs when the cloned gene is on a freely replicating multiple-copy plasmid or in the integrated form. Mapping of the cloned gene following integration of the 2 μ plasmid, and of the subclone in plasmid YRp7, showed it to be located on the left arm of chromosome XIV. Tetrad analysis of various crosses involving tow different strains carrying rad50-1 showed the mutation to map next to pet2 on chromosome XIV, and not on the right arm of chromsome IV, as previously published.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00382094
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