ISSN:
1432-072X
Keywords:
Arthrobacter oxidans
;
6-Hydroxy-d-nicotine oxidase
;
Gene expression
;
Nicotine regulon
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract In the gram-positive soil bacterium Arthrobacter oxidans d,l-nicotine induces specific flavoenzymes involved in the catabolism of the alkaloid. The delayed appearance of 6-HdNO activity as compared to that of the other enzymes of the nicotine regulon was investigated. Immunological methods and specific labeling of the 6-HdNO polypeptide with [14C]riboflavin showed that enzyme activity, occurrence of 6-HdNO antigen and covalent flavinylation of the 6-HdNO polypeptide all coincide with the stationary phase of cell growth. Northern blots, hybridized with an intragenic 6-HdNO probe, revealed a 6-HdNO-specific mRNA species of unique size only in stationary phase cells. Our results suggest that 6-HdNO is expressed from an independent transcription unit in a growth stage-dependent manner.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00447018
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