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Enzymic glucosylation of gibberellins (1984)

Knöfel, H. -D. ; Schwarzkopf, E. ; Müller, P. ; [et al.]

Springer

Journal of plant growth regulation 3 (1984), S. 127-140

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ISSN: 1435-8107

Source: Springer Online Journal Archives 1860-2000

Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition

Notes: Abstract Starting from the well-known conversion of exogenously applied free gibberellic acid (GA3) to its 3(O)-glucoside by intact immature fruits of runner beans (Phaseolus coccineus L.), a protein fraction has been prepared from this plant material possessing glucosylating activity towards GAs. This glucosyltransferase is located in the pericarp only and utilizes preferably UDP-glucose as a sugar donor. The product formed enzymically from GA3 and UDP-glucose could be identified by derivatization and comparison with the authentic compound to be GA3-3(O)-glucoside. Among 15 native or chemically modified GAs, the enzyme glucosylates only GA3 and to a lower extent GA7 and GA30, indicating a high enzyme specificity with regard to the A ring of gibberellins. The physiological significance of the enzymic GA3-3(O)-glucoside formation inPhaseolus coccineus is not clear, since this glucoside is not known to be endogenous in this plant. The enzyme preparation did not glucosylate substances of phenolic structure, such as hydroquinone, aesculetin, and quercetin. Glucosylation of GA3 was achieved also by enzyme preparations fromVigna sinensis and from cell suspension cultures ofDigitalis purpurea. A number of other plant materials showed no activity.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF02041998

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Quantification of rapid, transient increases in jasmonic acid in wounded plants using a monoclonal antibody (1993)

Albrecht, T. ; Kehlen, A. ; Stahl, K. ; [et al.]

Springer

Planta 191 (1993), S. 86-94

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ISSN: 1432-2048

Keywords: Avena ; Bryonia ; Jasmonate analysis ; (ELISA, monoclonal antibody) ; Jasmonic acid (woundinduced) ; Methyljasmonate (tritium labeled) ; Wounding

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract A monoclonal antibody (MAB JAH1-8-B4) for the analysis of 3R, 7R-jasmonic acid and its methyl ester is described. An IgG1(kappa) immunoglobulin, MAB JAH1-8-B4, was used to set up a competitive enzymelinked immunoassay employing 3R, 7R-jasmonate coupled to alkaline phosphatase as tracer. The assay has a linearity range (logit/log) between 50 fmol and 50 pmol (approx. 10 pg-10 ng) of 3R, 7R-methyljasmonate, the assay standard. A procedure combining prepurification of plant extracts by solid-phase extraction, followed by high-performance liquid chromatography and quantitation has been worked out, which uses 4 g of fresh plant material and has a detection limit between 0.2 and 0.4 μg of 3R, 7R-jasmonic acid (determined as its methyl ester) per kg of tissue, depending on the tissue. Internal standards of 3R, 7R-methyljasmonate, added to split samples during extraction as well as a second internal standard, 3R, 7R-methyljasmonate-[O-C3H3], added to all samples prior to methylation, served to correct for workup losses and for the monitoring of Chromatographie separations. Using this assay, it was found that levels of jasmonic acid rise immediately and transiently in the tissues analyzed as a consequence of wounding. These data provide further and direct evidence for the hypothesis that wound-induction of the plant defense reactions is mediated by endogenous jasmonates.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00240899

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