ISSN:
1432-2048
Keywords:
Key words: Beta (H+-ATPase)
;
H+-ATPase
;
Plasma membrane
;
Osmotic stress
;
14-3-3 protein
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract. Taking the binding of fusicoccin to plasma membranes as an indicator of complex formation between the 14-3-3 dimer and H+-ATPase, we assessed the effect of osmotic stress on the interaction of these proteins in suspension-cultured cells of sugar beet (Beta vulgaris L.). An increase in osmolarity of the cell incubation medium, accompanied by a decrease in turgor, was found to activate the H+ efflux 5-fold. The same increment was observed in the number of high-affinity fusicoccin-binding sites in isolated plasma membranes; the 14-3-3 content in the membranes increased 2- to 3-fold, while the H+-ATPase activity changed only slightly. The data obtained indicate that osmotic regulation of H+-ATPase in the plant plasma membrane is achieved via modulation of the coupling between H+ transport and ATP hydrolysis, and that such regulation involves 14-3-3 proteins.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s004250000347
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