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  • 1
    Electronic Resource
    Electronic Resource
    Preface (1999)
    Springer
    Cytotechnology 31 (1999), S. 1-2 
    Details
    ISSN: 1573-0778
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008198829774
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  • 2
    Electronic Resource
    Electronic Resource
    Three heterotrimeric laminins produced by human keratinocytes (2000)
    Springer
    Cytotechnology 33 (2000), S. 167-174 
    Details
    ISSN: 1573-0778
    Keywords: epiligrin ; kalinin ; keratinocyte ; laminin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Laminins are a family of glycoproteins composed of α,β and γ chains. Five α(α1-α5), three β (β1–β3) and twoγ (γ1 and γ2) chains have been cloned fromhuman and their replaceable assembly into heterotrimers producesthe variety of laminins. Reverse transcription-polymerase chainreaction of mRNAs showed that human keratinocytes express theα3, α5, β1, β3, γ1 andγ2 genes at high level among the ten cloned lamininchains. Western blot and immunoprecipitation of the cell lysatewith antiserum directed against mouse laminin-1(α1β1γ1) detected two trimers with thecomposition of αxβ1γ1 (probablylaminin-10 with the composition of α5β1γ1and αyβ1γ1. Meanwhile, antiserum directedagainst a synthetic peptide of human α3 detected onlyα3β3γ2 trimer (laminin-5). We thus show thatkeratinocytes produce three heterotrimeric laminins. We couldnot detect the assembly of α3 with β1 and γ1chains to form α3β1γ1 (laminin-6) in keratinocytes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008186912975
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  • 3
    Electronic Resource
    Electronic Resource
    Chromosomal assignment of the chicken NP220 gene encoding a large DNA-binding nuclear protein and its targeted disruption in chicken DT40 cells (2000)
    Springer
    Cytotechnology 33 (2000), S. 109-115 
    Details
    ISSN: 1573-0778
    Keywords: chicken gene ; chromosomal mapping ; matrin 3 ; NP220 ; nuclear matrix ; targeted integration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract NP220s form a family of DNA-binding nuclear proteinsoriginally found in human cell lines. Four isoformsof NP220 are produced in humans and mice probably byalternative splicing of two sequence units. Theyhave RNA-recognition and arginine/serine rich motivescommonly found in many nuclear proteins important forpre-mRNA processing. In order to analyze the functionof NP220s, its gene was disrupted in chicken cell lineDT40. For this, gemomic DNA clone of chicken NP220was isolated and the gene was localized tochromosome 4q2.7–q2.8. Chicken NP220 conserves MH1and zinc finger-like motives found in human and mouseNP220s. Despite its expression as a mRNA of 6 kb inwild type DT40 cells, disruption of the NP220gene did not impair the growth of DT40 cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008107227862
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  • 4
    Electronic Resource
    Electronic Resource
    The differentiation-dependent inhibition of SV40 early promoter/enhancer activity in 3T3-L1 cells is mediated through promoter and not enhancer sequences (1993)
    Springer
    Cytotechnology 11 (1993), S. 175-182 
    Details
    ISSN: 1573-0778
    Keywords: adipocyte ; competitive gene transfection ; differentiation ; fibroblast ; viral promoter ; 3T3-L1 cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Using a plasmid bearing chloramphenicol acetyltransferase (CAT) gene controlled by Simian virus 40 (SV40) early promoter/enhancer complex (pA0cat), we analyzed functional enhancer motifs in 3T3-L1 fibroblast and adipocyte cells. Deletion mutant series of pA0 at the enhancer complex showed that gene expression both in fibroblast and adipocyte cells was dependent on a similar set of enhancer motifs. When pA0 was introduced into 3T3-L1 fibroblasts and the cells were induced to differentiate into adipocytes, CAT activity expressed in fibroblasts was suppressed. Experiments with the deletion mutants at the enhancer complex showed that the suppression was not related to any enhancer motif, and CAT activity was observed with a plasmid having only the promoter sequence. When pA0cat was co-transfected with excess of promoter sequence, the suppression in adipocytes was counteracted. This suggested that negativetrans-acting factors of the promoter sequence were responsible for the suppression in adipocytes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00749867
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  • 5
    Electronic Resource
    Electronic Resource
    Potential molecular chaperones involved in laminin chain assembly (1997)
    Springer
    Cytotechnology 25 (1997), S. 173-182 
    Details
    ISSN: 1573-0778
    Keywords: Bip ; calnexin ; GRP94 ; HSP70 ; laminin ; molecular chaperone ; protein-disulfide isomerase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract To explore potential molecular chaperones involved in the intracellular assembly of laminin chains, bovine aortic endothelial cells were treated with a thiol cleavable divalent cross-linking reagent, dithio-bis-(succinimidylpropionate), and cellular proteins cross-linked to laminin chains were co-immunoprecipitated with anti-laminin antiserum. Sodium dodecylsulfate (SDS) gel electrophoresis of the precipitate under reducing condition showed polypeptides with estimated sizes of 80, 60 and 50 kDa together with laminin chains. Two dimensional electrophoresis, in which non-reducing and reducing SDS electrophoresis were combined, suggested that many molecules of these polypeptides were cross-linked to each laminin chain. Sepharose CL-4B beads conjugated with E8 fragment of mouse laminin-1 was prepared. Affinity chromatography with the beads of microsomal proteins from rat liver showed that Bip and HSP70 associated to laminin chains and dissociated upon ATP hydrolysis. Protein-disulfide isomerase also showed affinity to the column. GRP94 and calnexin showed strong affinity and were washed out only with a detergent solution. Thus, many molecular chaperones are suggested to be involved in the intracellular assembly of laminin chains.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1007920018109
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  • 6
    Electronic Resource
    Electronic Resource
    De novo adipogenesis for reconstructive surgery (1999)
    Springer
    Cytotechnology 31 (1999), S. 29-35 
    Details
    ISSN: 1573-0778
    Keywords: adipogenesis ; adipocyte determination and differentiation-dependent factor 1 (ADD1) ; basement membrane ; basic fibroblast growth factor (bFGF) ; CCAAT-enhancer-binding proteins (C/EBPs) ; peroxisome proliferator activated receptors (PPARs) ; reconstructive surgery
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Autografting of lost soft tissue is an important subject of the plastic and reconstructive surgery and autograft of fat pads has been only technique for this goal. However, the results are disappointing because of absorption of the grafts with time. Adipoblasts or adipocyte precursor cells distribute widely in connective tissues and they can proliferate and mature into adipocytes even in the adult body. In experiments using mice, we found that de novo adipogenesis of endogenous precursor cells can be induced by injecting reconstituted basement membrane, Matrigel, supplemented with more than 1 ng/ml of bFGF. This adipogenesis was reproducibly induced by subcutaneous injection over the chest, lateral abdomen or head. Adipogenesis was induced even in ear cartilage or in muscle. To evaluate the possibility of future application of this de novo adipogenesis to plastic and reconstructive surgery, we have reviewed updated knowledge of the adipogenesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008055702129
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  • 7
    Electronic Resource
    Electronic Resource
    Differential expression of mRNAs encoding laminin chain variants during in vitro development of mouse blastocysts (1999)
    Springer
    Cytotechnology 31 (1999), S. 185-193 
    Details
    ISSN: 1573-0778
    Keywords: blastocyst ; egg cylinder ; embryogenesis ; implantation ; laminin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Expression of mRNAs encoding ten laminin chain variants was studied by reverse transcription-polymerase chain reaction (RT-PCR) of mRNA extracted from cultured mouse blastocysts. α1, β1 and γ1 mRNAs, of which products form laminin-1, were the only chains expressed in blastocysts before hatching. α4 and α5 mRNAs were additionally expressed after attachment of blastocysts to culture dishes. α2 and β2 mRNAs started to be expressed at the stage of allantois formation. Among ten laminin chains, only the mRNA encoding components of laminin-5 (α3, β3 and γ2) was missing in embryos up to the stage of allantois formation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008084508489
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  • 8
    Electronic Resource
    Electronic Resource
    Reconstituted basement membrane potentiates in vivo adipogenesis of 3T3-F442A cells (1999)
    Springer
    Cytotechnology 31 (1999), S. 215-220 
    Details
    ISSN: 1573-0778
    Keywords: adipogenesis ; basement membranes ; differentiation ; Matrigel ; preadipocyte
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Adipocytes forming fat pad in vivo are surrounded by well developed basement membranes. Synthesis of basement membrane is enhanced during in vitro differentiation of preadipocyte line. In order to know the role of basement membrane in adipogenesis in vivo, we injected 3T3-F442A preadipocytes subcutaneously into nude mice together with or without the reconstituted basement membrane, Matrigel. Histological sections of the fat pads newly formed by injecting the cell alone showed dense population of immature adipocytes and microvessels within 2 weeks and they matured rapidly. In contrast, injection of the cells together with Matrigel showed sparse adipocytes after 2 weeks and they matured slowly over the period of 6 weeks. Quantification of the process by measuring the weight, DNA content, triglyceride content and glycerophosphate dehydrogenase (GPDH) activity of the fat pads showed that injection of the cell alone resulted in early maturation of adipose tissue with fewer adipocytes while the presence of Matrigel decelerated but potentiated the maturation of adipose tissue with 2 fold contents of DNA, triglyceride and GPDH activity. We thus showed that reconstituted basement membrane (Matrigel) supported the survival and maturation of adipocytes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008198731341
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  • 9
    Electronic Resource
    Electronic Resource
    Reversible effects of dibutyryl cAMP on laminin and type IV collagen secretion from retinoic acid treated F9 cells (1985)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 125 (1985), S. 263-272 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The effects of dibutyryl cAMP on the differentiation of embryonal carcinoma F9 cells were studied mainly using the secretion of laminin and type IV collagen as the marker. For this purpose, F9 cells were labeled with 35Smethionine and radioactive proteins in the medium were analyzed by SDS-polyacrylamide gel electrophoresis. Treatment of F9 cells with retinoic acid alone induced differentiation into cells secreting type IV collagen. The combination of retinoic acid and dibutyryl cAMP stimulated laminin secretion in addition to type IV collagen secretion. This effect of dibutyryl cAMP was observed only 16 h after adding dibutyryl cAMP. Immunofluorescence staining demonstrated that the majority of the cells in culture were converted into cells secreting laminin under these conditions. In contrast to the irreversible effect of retinoic acid, the effect of dibutyryl cAMP on laminin and type IV collagen secretion was reversible at least during the first 5 days of maintaining cells in the medium containing retinoic acid plus dibutyryl cAMP. Removal of dibutyryl cAMP from the culture medium decreased the protein secretion to the basal levels within 2 days. This reversibility was not due to a change in cell number. An in vitro translation assay also suggested the reversible effect of dibutyryl cAMP on the levels of laminin mRNA. Coinciding with variations of the protein secretion, a reversible and homogeneous change in the morphology of retinoic acid generated F9 cells was observed by dibutyryl cAMP.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041250214
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  • 10
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    Bipartite nuclear localization signal of matrin 3 is essential for vertebrate cells (2007)
    Elsevier
    Details
    Publication Date: 2007-03-01
    Print ISSN: 0006-291X
    Electronic ISSN: 1090-2104
    Topics: Biology , Chemistry and Pharmacology , Physics
    Published by Elsevier
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