ISSN:
0368-1467
Keywords:
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Information on a reliable method for determining the nutritive value of existing and experimental peptones is lacking in the literature. To overcome this handicap, a method for comparative evaluation of microbiological peptones has been developed.The method is based on measuring the growth of eight carefully selected, fastidious, test cultures. The cultures are grown in presence and absence of yeast extract in an inorganic basal medium containing test peptone and a low and high level of glucose.Statistical analysis of replicate peptone evaluations of six peptones, conducted over a period of six months, shows that 95 per cent confidence limits for this method are ± 2·5 to 7·9 per cent of the mean.Four criteria are employed for determining the overall performance of 12 widely used, commercial peptones.The method is very sensitive for detecting differences between batches of commercial peptones. With the aid of this method a new peptone ‘N-Z-Amine YT’ has been developed. Considering performance and relative cost, this peptone is the most economical of the peptones evaluated.
Additional Material:
2 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jbmte.390010110
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