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  • 1
    Electronic Resource
    Electronic Resource
    Genetically engineered resistance to potato virus X in four commercial potato cultivars (1995)
    Springer
    Plant cell reports 15 (1995), S. 91-96 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The genes for the capsid protein (CP) and the 8K movement protein of PVX were introduced into potato (Solanum tuberosum L.) and expressed under the control of CaMV 35S promoter using a binary vector andAgrobacterium tumefaciens. Four commercial potato cultivars (Russet Burbank, Shepody, Desirée and Bintje) have been efficiently transformed. Eleven independent transgenic clones, with CP expression levels higher than 0.05% of the soluble leaf proteins, were analyzed for resistance to inoculation with PVX (5 and 50µg/ml). The resistance of the transgenic plants to PVX was observed with the lower titer of virus inoculation (5 µg/ml) but not with higher titer (50 µg/ml). A significant reduction in the accumulation of virus in the inoculated transgenic potato plants has been observed under greenhouse and field conditions. Furthermore, the CP gene is very stable and is transferred to new plants originated from stem cuttings or from tubers. The transgenic plants appeared to be phenotypically identical to the nontransformed controls.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01690261
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Expression of the PVYO coat protein (CP) under the control of the PVX CP gene leader sequence: protection under greenhouse and field conditions against PVYO and PVYN infection in three potato cultivars (1997)
    Springer
    Theoretical and applied genetics 94 (1997), S. 287-292 
    Details
    ISSN: 1432-2242
    Keywords: Key words Potato virus Y ; Potato virus X ; Transgenic plants ; Translational enhancer ; CPMR
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Coat protein-mediated resistance (CPMR), resistance conferred as a result of the expression of viral coat proteins in transgenic plants, has been illustrated to be an effective way of protecting plants against several plant viruses. Nonetheless, consistent protection has not been achieved for transgenic plants expressing the coat protein of potato virus Y (PVY), the type member of the potyvirus family. In this report, three different potato cultivars were transformed with a chimeric construct consisting of the capsid protein (CP) coding sequences of PVY flanked by the AUG codon and the translational enhancer from the coat protein gene of potato virus X (PVX). These cultivars were shown to express high levels of PVY CP and confer a high degree of protection against PVYo and PVYN under both greenhouse and field conditions. In addition, transgenic plants infected with potato virus A (PVA), a related potyvirus, exhibited a delay in virus accumulation, which could be easily overcome with increasing virus concentrations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050412
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    Paper (German National Licenses)
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