ISSN:
0192-253X
Keywords:
Transformation
;
electroporotion
;
gene targetting
;
antisense RNA
;
complementation
;
Life and Medical Sciences
;
Genetics
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
Notes:
DNA mediated transformation is a critical technique for uniting genetic, molecular genetic and reverse genetic approaches to a wide range of problems in cell and molecular biology. In Dictyostelium, there is now the capability not only to manipulate DNA sequences in vitro and put them back into the cell, but also to alter the sequences of endogenous chromosomal genes through high frequency homologous recombination. This means that the range of gene manipulation techniques that have made yeast such a useful system should now be applicable in Dictyostelium. For studying problems such as cellular motility, morphogenesis, and gene regulation, few organisms have the combination of features offered by Dictyostelium.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/dvg.1020110510
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