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  • 1
    Electronic Resource
    Electronic Resource
    Electrophoretic separation of human lymphocytes by means of immunomicrospheres (1982)
    Weinheim : Wiley-Blackwell
    Electrophoresis 3 (1982), S. 300-305 
    Details
    ISSN: 0173-0835
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Immuno-labeling of human peripheral blood lymphocytes with either polyglutaraldehyde or polyacrolein microspheres was shown by analytical electrophoresis to generate two electrokinetically distinct sub-populations of lymphocytes. By means of free-flow electrophoresis, the high mobility fraction was found to consist predominantly of T lymphocytes using an anti-T cell antibody, while the low mobility fraction consisted of lymphocytes labeled with immunomicrospheres, identifying these cells as B lymphocytes. The immunomicrosphere-induced reduction of B-cell mobility below that of the T cells thus allowed the enrichment and recovery of viable T cells in preparative quantities by free-flow electrophoresis.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150030510
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  • 2
    Electronic Resource
    Electronic Resource
    Electrophoretic cell separation using polyacrolein microspheres (1982)
    Weinheim : Wiley-Blackwell
    Electrophoresis 3 (1982), S. 109-113 
    Details
    ISSN: 0173-0835
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: The electrophoretic mobility of a number of microspheres of homo- or copolymerized acrolein has been studied. It was found that increasing amounts of methacrylic acid increased the negative mobility of the microspheres, whereas reaction of the microspheres with 1,6-diaminohexane decreased the negative mobility. When coated with antibody and specifically bound to cells, these microspheres were found to alter the target cells' electrophoretic mobility. This process allowed the separation of synthetic mixtures of microsphere-labeled and unlabeled cell populations. Over 90 % of the recovered cells were found in fractions that were essentially either pure labeled or unlabeled cells.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150030210
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    Paper (German National Licenses)
    Fulltext
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