ISSN:
1612-1112
Keywords:
Column liquid chromatography
;
Isolation of natural products
;
Solid phase extraction
;
Chemically bonded phase
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary Esculin (ESC) and rutin (RUT) have been simultaneously isolated from pharmaceutical natural materials by solid phase extraction (SPE). Determination of both substances was performed by reversed phase high performance liquid chromatography (RPHPLC) with UV detection. Optimization of the separation conditions showed that simultaneous isolation and determination of rutin and esculin from pharmaceutical material was possible. The recovery obtained was not lower than 95±2%.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02277516
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