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  • 1
    Electronic Resource
    Electronic Resource
    Physical and genetic analyses of IncI2 plasmid R721: Evidence for the presence of shufflon
    Amsterdam : Elsevier
    Plasmid 23 (1990), S. 248-251 
    Details
    ISSN: 0147-619X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0147-619X(90)90057-J
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  • 2
    Electronic Resource
    Electronic Resource
    The nucleotide sequence and expression of the gene for the 32-kDa quinone-binding protein from rice (Oryza sativa L.)
    Amsterdam : Elsevier
    Plant Science 59 (1989), S. 95-99 
    Details
    ISSN: 0168-9452
    Keywords: chloroplast gene ; psbA ; rice ; transcription
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0168-9452(89)90013-7
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  • 3
    Electronic Resource
    Electronic Resource
    Maintenance of chloroplast-derived sequences in the mitochondrial DNA of Gramineae (1997)
    Springer
    Current genetics 32 (1997), S. 413-419 
    Details
    ISSN: 1432-0983
    Keywords: Key words Chloroplast-derived (ct-derived) sequence ; Mitochondrial DNA (mtDNA) ; Gramineae ; Variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Evidence for the transfer of DNA from the chloroplast to the mitochondrion has been reported in many higher plants and, in most cases, the transferred chloroplast genes do not have the ability to encode functional products as a consequence of base substitutions and/or multiple rearrangements. We reported previously that the sequence of one end of a chloroplast-derived (ct-derived) fragment of DNA that contained the rps19 and trnH genes has been maintained in most gramineous plants and that its presence seems to be correlated with gene expression in this region. In the present study, we have investigated whether or not the ct-derived sequences in mitochondrial DNA (mtDNA) from some gramineous plants and species of Oryza are conserved, and whether or not such conservation is related to gene expression in these regions. We identified two junctions between ct-derived and mitochondrial sequences that were conserved among some gramineous plants. Around these regions, we found a ct-derived gene for tRNA and the promoter of a mitochondrial gene on the ct-derived sequences, respectively, and these regions were transcribed through the junctions. This result indicates that the junctions and/or regions that are transcribed and functional in mitochondria have been strongly conserved and maintained during their evolution. In Oryza, some junctions between ct-derived and mitochondrial sequences were conserved and other junctions were not. These variations seem to have been caused by deletions and/or rearrangements, and appear to be specific to the type of genome. In the case of Oryza, the timing of deletions and/or rearrangements of ct-derived sequences is likely to have coincided with the divergence of the various genome types.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002940050296
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  • 4
    Electronic Resource
    Electronic Resource
    Detailed analyses of chloroplast and mitochondrial DNAs from the hybrid plant generated by asymmetric protoplast fusion between radish and cabbage (1997)
    Springer
    Plant cell reports 16 (1997), S. 479-484 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In a previous report, intergeneric somatic hybrids between red cabbage (Brassica oleracea L. var. capitata) and radish (Raphanus sativus L. cv. Shougoin) were produced by protoplast fusion. Plant morphology, chromosome number, isozyme patterns, and SmaI cleavage pattern of chloroplast DNA indicated that the hybrid plants have the red cabbage nucleus and the radish chloroplasts. In this report, we analyzed the organization of chloroplast and mitochondrial DNAs from this hybrid using Southern hybridization. The restriction patterns of almost all regions of the chloroplast DNA from the hybrid were similar to that of radish, except for one region near the rps16 gene, which encodes the chloroplast ribosomal protein S16. In contrast to chloroplast DNA, the restriction pattern of mitochondrial DNA from the hybrid was quite different from that of the parents.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01092770
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  • 5
    Electronic Resource
    Electronic Resource
    Detailed analyses of chloroplast and mitochondrial DNAs from the hybrid plant generated by asymmetric protoplast fusion between radish and cabbage (1997)
    Springer
    Plant cell reports 16 (1997), S. 479-484 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In a previous report, intergeneric somatic hybrids between red cabbage (Brassica oleracea L. var.capitata) and radish (Raphanus sativus L. cv. Shougoin) were produced by protoplast fusion. Plant morphology, chromosome number, isozyme patterns, andSma1 cleavage pattern of chloroplast DNA indicated that the hybrid plants have the red cabbage nucleus and the radish chloroplasts. In this report, we analyzed the organization of chloroplast and mitochondrial DNAs from this hybrid using Southern hybridization. The restriction patterns of almost all regions of the chloroplast DNA from the hybrid were similar to that of radish, except for one region near therps16 gene, which encodes the chloroplast ribosomal protein S16. In contrast to chloroplast DNA, the restriction pattern of mitochondrial DNA from the hybrid was quite different from that of the parents.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01092770
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  • 6
    Electronic Resource
    Electronic Resource
    Intergeneric somatic hybridization of rice (Oryza sativa L.) and barley (Hordeum vulgare L.) by protoplast fusion (1998)
    Springer
    Plant cell reports 17 (1998), S. 362-367 
    Details
    ISSN: 1432-203X
    Keywords: Key words Intergeneric somatic hybrid ; Oryza sativa L. ; Hordeum vulgare L. ; Chromosome ; Recombination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An intergeneric somatic hybrid was obtained upon fusion of protoplasts of rice and barley. Protoplasts isolated from suspension cultures of rice cells were fused by electrofusion with protoplasts that had been isolated from young barley leaves. Some of the resultant calli formed green spots and shoots. Only one shoot formed roots, and it was subsequently successfully transferred to soil in a greenhouse. Its morphology closely resembled that of the parental rice plant. Cytological analysis indicated that the plant had both small chromosomes from rice and large chromosomes from barley. Southern hybridization analysis with a fragment of the tryptophan B (trpB) gene revealed both a rice-specific band and a barley-specific band. Mitochondrial (mt) and chloroplast (cp) DNAs were also analyzed using the same method. The plant was shown to contain novel mitochondrial and chloroplast sequence rearrangements that were not detected in either of the parents.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050407
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  • 7
    Electronic Resource
    Electronic Resource
    Variations in chloroplast DNA from rice (Oryza sativa): differences between deletions mediated by short direct-repeat sequences within a single species (1993)
    Springer
    Theoretical and applied genetics 86 (1993), S. 579-584 
    Details
    ISSN: 1432-2242
    Keywords: Oryza sativa ; Indica ; Chloroplast DNA ; Deletion ; Direct repeat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In a previous study, we compared chloroplast DNAs (ctDNAs) from four species ofOryza and detected two independent deletions of DNA fragments in the ctDNAs (Kanno and Hirai 1992a). These deletions were genotype-specific variations. Since short direct-repeat sequences were detected at the borders of both deletions, the deletions were apparently the result of intramolecular recombination mediated by these direct-repeat sequences. In the present study, we examined whether or not this type of variation exists within a single species. Ishii et al. demonstrated three types of ctDNA inO. Sativa (1988), and the source of the variations that they identified seemed to be deletions. We determined the precise locations of the deletions and the sequences around them. As expected, our results showed that these variations were the results of deletions that were mediated by short direct-repeat sequences. While the deletions that had been found previously were located on spacer regions, those found in this study were located within open reading frames (ORFs). Northern hybridization analysis showed that one of the ORFs was-transcribed. In the case of this deletion, the amino acid sequence encoded by the C-terminal region of the ORF was altered and the short inverted-repeat sequences downstream of the ORF were deleted. In addition, there were other short inverted-repeat sequences downstream of the altered ORF.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00838712
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  • 8
    Electronic Resource
    Electronic Resource
    The physical map of the chloroplast DNA from Asparagus officinalis L. (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 10-14 
    Details
    ISSN: 1432-2242
    Keywords: Key words Chloroplast DNA (ctDNA)  ;  Physical map  ;  Garden asparagus  ;  Asparagus officinalis L.  ;  Chloroplast genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The genus Asparagus consists of 100–300 species of both dioecious and hermaphrodite plants. Since there are diploid, tetraploid, and hexaploid plants in this genus, RFLP (restriction fragment length polymorphism) analysis of chloroplast DNA (ctDNA) is suitable for examining the phylogenetic relationships. We have constructed a physical map of the ctDNA of garden asparagus (A. officinalis L. cv ‘Mary Washington 500 W’) using five restriction endonucleases, namely, BamHI, PstI, SalI, HindIII, and XhoI. Asparagus ctDNA was digested with restriction enzymes and cloned into plasmid and λ phage vectors, and a clone bank was constructed that covered 70% of the genome. A physical map was constructed by Southern hybridization of total DNA from asparagus with homologous and heterologous probes. The asparagus ctDNA was about 155 kb long and it contained two inverted repeats (23 kb each) separated by a large single-copy region (90 kb) and a small single-copy region (19 kb). Fifteen genes, encoding photosynthesis-related proteins, rDNAs, and tRNAs, were localized on the physical map of asparagus ctDNA. Comparing the length and the gene order of asparagus ctDNA with that of other plants, we found that asparagus ctDNA was similar to tobacco ctDNA but different from rice ctDNA. The restriction patterns of the ctDNAs from several varieties of A. officinalis and three species of Asparagus were analyzed. The restriction patterns of the varieties of A. officinalis were very similar, but polymorphisms were detected among the three species of Asparagus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222945
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  • 9
    Electronic Resource
    Electronic Resource
    Production and analysis of plants that are somatic hybrids of barley (Hordeum vulgare L.) and carrot (Daucus carota L.) (1997)
    Springer
    Theoretical and applied genetics 94 (1997), S. 221-226 
    Details
    ISSN: 1432-2242
    Keywords: Key wordsHordeum vulgare L. ; Daucus carota L. ; Interfamilial somatic hybrid ; Low temperature ; Recombination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In order to obtain plants that were somatic hybrids of barley (Hordeum vulgare L.) and carrot (Daucus carota L.), we fused protoplasts that had been isolated from 6-month-old suspension cultures of carrot cells with protoplasts isolated from barley mesophyll by electrofusion. After culture for 1 month at 25°C , the cells were cultured for 5 weeks at 4°C , and were then returned to 25°C for culture on a shoot-inducing medium. Three plants (nos. 1, 2 and 3) were regenerated from the cells. The morphology of the regenerated plants closely resembled that of the parental carrot plants. A cytological analysis of callus cultures induced from these plants indicated that most of the cells had about 24 chromosomes, fewer than the sum of the numbers of parent chromosomes which was 32. Southern hybridization analysis with fragments of the rgp1 gene used as probe showed that the regenerated plants contained both barley and carrot genomic DNA. Chloroplast (ct) and mitochondrial (mt) DNAs were also analyzed with several probes. The ctDNA of the regenerated plants yielded hybridization bands specific for both barley and carrot when one fragment of rice ctDNA was used as probe. Furthermore, the regenerated plants yielded a barley specific band and a novel band with another fragment of rice ct DNA as a probe. One of the regenerated plants (no. 1) yielded a novel pattern of hybridized bands of mt DNA (with an atp6 probe) that was not detected with either of the parents. These results indicated that the regenerated plants were somatic hybrids of barley and carrot and that recombination of both the chloroplast genomes and the mitochondrial genomes might have occurred.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050403
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  • 10
    Electronic Resource
    Electronic Resource
    Comparative studies of the structure of chloroplast DNA from four species of Oryza: cloning and physical maps (1992)
    Springer
    Theoretical and applied genetics 83 (1992), S. 791-798 
    Details
    ISSN: 1432-2242
    Keywords: Wild rice ; Chloroplast DNA ; Clone bank ; Physical map ; Deletion/insertion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Chloroplast DNAs (ctDNAs) were prepared from the mature green leaves of three species in the genus Oryza, namely, O. punctata (genome type BB), O. offici-nalis (CC), and O. australiensis (EE). After digestion with restriction enzymes, ctDNAs were cloned into a lambda phage vector and overlapping clone banks of the entire chloroplast genome from each of the three Oryza species were obtained. BamHI and PstI restriction maps of the ctDNAs were constructed, and the structures of the ctDNAs from O. sativa and the other three Oryza species were compared. Two types of variation were noted: the gain or loss of restriction sites, and deletion or insertion of nucleotides. We detected two independent deletions in the BamHI-3/PstI-3 fragment of O. punctata and in the BamHI-5/PstI-11 fragment of O. officinalis, each of which was shorter than the respective fragment from O. sativa, and the deletions were located in spacer regions. Short direct-repeat sequences were detected at the border of both deletions, indicating that these deletions were results of intramolecular recombination mediated by these direct repeats. Further analysis on distribution of those deletions among 15 Oryza species revealed that the deletions found in this study represent genotype-specific variations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226699
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