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  • 1
    Electronic Resource
    Electronic Resource
    Expression of heat-shock protein 47 in mouse liver (1996)
    Springer
    Cell & tissue research 284 (1996), S. 341-346 
    Details
    ISSN: 1432-0878
    Keywords: Key words: Heat-shock protein 47 ; Nonparenchymal liver cells ; Stellate cells ; Fibrosis ; Mouse (ICR)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Expression of heat-shock protein 47 in intact and fibrotic liver and in hepatic constituent cells was investigated in mice. Immunohistochemical study of intact liver and Western blot analysis of the protein from isolated liver cells revealed that stellate cells and smooth muscle cells of interlobular vessels, but not hepatocytes, Kupffer cells, or endothelial cells, expressed heat-shock protein 47. The protein was found in both vitamin-A-storing stellate cells and myofibroblast-like cells. The amount of the protein in cultured stellate cells was reduced by dexamethasone but was not regulated by quercetin, transforming growth factor β, interferon γ, or retinoic acid. In CCl4-treated or bile-duct-ligated mouse liver, the number of cells positive for heat-shock protein 47 markedly increased in the centrilobular area or around the periportal area, respectively, and the level of heat- shock protein 47 also increased.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004410050594
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  • 2
    Electronic Resource
    Electronic Resource
    Localization and ultrastructure of pulmonary dendritic cells during delayed-type hypersensitivity reactions in mice (1999)
    Springer
    Cell & tissue research 297 (1999), S. 485-492 
    Details
    ISSN: 1432-0878
    Keywords: Key words Antigen-presenting cells ; T cells ; Bronchiole ; Lung ; Mouse (BALB/c)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  Dendritic cells (DCs) are widely distributed in the airways and can serve as potent antigen-presenting cells. To clarify their involvement in the cell-mediated immune responses of the lung, we immunohistochemically investigated their distribution and kinetics during pulmonary delayed-type hypersensitivity (DTH) reactions induced in sensitized mice by intratracheal instillation of hapten. Cellular infiltrate appeared around the bronchiole and its accompanying blood vessel at 12 h after elicitation and progressively expanded by 48 h. As quantitated by computer-assisted morphometry, I-A+ DCs and CD4+ Th cells significantly increased in number around the bronchiole to a maximum at 24 h, whereas F4/80+ macrophages were predominantly accumulated around the accompanying vessel with a peak at 48 h. Serial-section analysis revealed that DCs were colocalized with Th cells in the inflamed peribronchiolar tissue. Immunoelectron microscopy demonstrated that DCs found inside and around the capillaries and venules of peribronchiolar interstitium displayed round forms, indicating their emigration from here, while those situated far from the microvessels were elongated, often in close apposition to the lymphocytes. Mitosis of DCs was rarely seen. The present results suggest that peribronchiolar accumulation of DCs resulting from accelerated influx of blood-borne immature DCs and the interaction with T cells at the application site may play inducing roles in the development of pulmonary DTH reactions by enhancing the recruitment of macrophages.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004410051375
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  • 3
    Electronic Resource
    Electronic Resource
    Expression of vascular endothelial growth factor by granulated metrial gland cells in pregnant murine uteri (2000)
    Springer
    Cell & tissue research 300 (2000), S. 285-293 
    Details
    ISSN: 1432-0878
    Keywords: Angiogenesis Decidua Metrial gland Mouse (C57BL/6Cr)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Granulated metrial gland (GMG) cells are a characteristic uterine component belonging to a natural killer cell lineage. This study is aimed at revealing their kinetic and spatial relationship with vascular growth during pregnancy and the expression of vascular endothelial growth factor (VEGF). GMG cells and blood vessels were identified by periodic-acid-Schiff-reagent (PAS)-stained granules and positive staining for factor-VIII-related antigen, respectively. GMG cells were widely distributed in the decidua and metrial gland and showed a numerical increase with a peak at day 13 in parallel with the increase of vascular density. Preceding the maximal vascular development at day 13, microvessels with a narrow lumen representative of neovascularization prevailed at days 7–9, and the VEGF content in the decidua/metrial gland was significantly elevated at days 7–13 concurrently with mRNA expression. By immunolight microscopy combined with PAS staining, GMG cells with PAS-stained granules were positive for VEGF. Immunoelectron microscopy demonstrated that immunoreactions were diffuse in the cytoplasm but not localized in the granules. In contrast, fibroblast-like stromal cells were negative. These data indicate that GMG cells express VEGF and may play inducing roles in uterine neovascularization during pregnancy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004410000198
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  • 4
    Electronic Resource
    Electronic Resource
    Quantitative analysis of rod-cored vesicles and dense granules of large granular lymphocytes in the liver, spleen, and peripheral blood of rats (1994)
    Springer
    Cell & tissue research 276 (1994), S. 187-195 
    Details
    ISSN: 1432-0878
    Keywords: Rod-coredvesicles ; Granules ; Lymphocytes ; Liver ; Electron microscopy ; Rat (Fischer F344/NCR)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Large granular lymphocytes (LGL) comprise a natural defense system in the liver and exert an inhibitory effect on tumor cell metastasis. In order to demonstrate the maturation of LGL in the liver from the morphological aspect, we evaluated electron-microscopically the frequency of 0.2 μm vesicles (rod-cored and “empty” vesicles) and dense granules in LGL from the liver, spleen, and peripheral blood of the rat. Both of these cell organelles are characteristic to LGL and may relate to natural killer-mediated cytolysis. On the average, there were 12.7 of the 0.2 μm vesicles and 4.3 rod-cored vesicles (RCV) per cell section in the liver, 6.6 0.2 μm vesicles and 1.6 RCV in the spleen, and 8.6 0.2 μm vesicles and 0.9 RCV in the peripheral blood. The number of 0.2 μm vesicles per cell section ranged from 0 to 19 with the exception of a few higher instances. Therefore, LGL were divided into vesicle-rich(〉9 0.2 μm vesicles per cell section) and vesicle-poor (〈8 per cell section) populations. Hepatic LGL consisted mainly of a vesicle-rich population while splenic LGL consisted mainly of a vesicle-poor population, and peripheral blood contained equal proportions of both populations. In addition to diversity with regard to the number of 0.2 μm vesicles, LGL obtained from various organs also displayed heterogeneity in the number and size of dense granules. Since the number of dense granules per cell section usually ranged from 1 to 13, LGL were diveded into 2 populations, i.e., LGL with many (〉7 per cell section) granules and those with a few(〈6 per cell section) granules. Specifically, splenic LGL had a few small (average diameter, less than 400 nm) dense granules, while sections of LGL from the liver and peripheral blood displayed many small dense granules and a few large (〉400 nm) ones, respectively, in addition to the populations seen in the spleen. Thus, the present study has demonstrateda difference in the distribution of 0.2 μm vesicles in LGL based on the tissue of origin. The present study has revealed the difference in the distribution of 0.2 μm vesicles of LGL by tissue and indicated that immature LGL are predominant in the spleen, while hepatic LGL are generally more mature as defined by the number of vesicles. These data suggest that the microenvironment of the liver may contribute to the increased expression of these vesicles in LGL.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00354799
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  • 5
    Electronic Resource
    Electronic Resource
    Expression of neural cell adhesion molecule (N-CAM) in perisinusoidal stellate cells of the human liver (1995)
    Springer
    Cell & tissue research 283 (1995), S. 159-165 
    Details
    ISSN: 1432-0878
    Keywords: Key words: Cell adhesion molecules ; neuronal ; Stellate cells ; Liver ; Immunohistochemistry ; Human
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Neural cell adhesion molecule (N-CAM) is distributed in most nerve cells and some non-neural tissues. The present immunohistochemical study has revealed, for the first time, the expression of N-CAM in perisinusoidal stellate cells of the human liver. Liver specimens were stained with monoclonal antibody against human Leu19 (N-CAM) by a streptoavidin-biotin-peroxidase-complex method. Light- and electron-microscopic analyses have shown that N-CAM-positive nerve fibers are distributed in the periportal and intermediate zones of the liver lobule. Perisinusoidal stellate cells in these zones are also positive for N-CAM. N-CAM is expressed on the surface of the cell, including cytoplasmic projections. Close contact of N-CAM-positive nerve endings with N-CAM-positive stellate cells has been observed. On the other hand, stellate cells in the centrilobular zone exhibit weak or no reaction for N-CAM. Perivascular smooth muscle cells and fibroblasts in the portal area and myofibroblasts around the central veins are negative for N-CAM. The present results indicate that the perisinusoidal stellate cells in the periportal and intermediate zones of the liver lobule characteristically express N-CAM, unlike other related mesenchymal cells, and suggest that the intralobular heterogeneity of N-CAM expression by stellate cells is related to the different maturational stages of these cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004410050524
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  • 6
    Electronic Resource
    Electronic Resource
    A new type of phosphoglycolipid fromPseudomonas cepacia (1988)
    Springer
    Current microbiology 16 (1988), S. 217-219 
    Details
    ISSN: 1432-0991
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract A new type of phosphoglycolipid was found inPseudomonas cepacia. A ratio of glucosamine/phosphoric acid/polar fatty acid/nonpolar fatty acid was resolved to be 1 ∶ 1 ∶ 1 ∶ 1, and the molecular weight of the phosphoglycolipid was determined to be 777 by mass spectrometry. An amino group of the glucosamine was free, and both a phosphoric acid and two kinds of fatty acids were bound directly to the glucosamine.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01568532
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  • 7
    Electronic Resource
    Electronic Resource
    Distribution and morphological characteristics of the pit cells in the liver of the rat (1983)
    Springer
    Cell & tissue research 233 (1983), S. 485-505 
    Details
    ISSN: 1432-0878
    Keywords: Liver ; Pit cell ; Cytoplasmic granules ; Vesicles ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Pit cells, on which almost no further contributions have been presented since the first report by Wisse et al. (1976), are described in detail in the rat liver. These cells show several characteristic features: 1) “rod-cored vesicles”, a new type of vesicular inclusion observed first in our study; 2) electron-dense granules, which we consider to arise from multivesicular bodies by the accumulation of dense material; and 3) well-developed pseudopodia. Although these features clearly differentiate pit cells from conventional lymphocytes, these two cell types display similarities (i) in a number of ultrastructural features, (ii) in the pattern of their intralobular distribution, and (iii) in their presence in the spleen and peripheral blood.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00212219
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  • 8
    Electronic Resource
    Electronic Resource
    Morphological study of endothelin-1-induced contraction of cultured hepatic stellate cells on hydrated collagen gels (1996)
    Springer
    Cell & tissue research 286 (1996), S. 477-486 
    Details
    ISSN: 1432-0878
    Keywords: Key words: Hepatic stellate cells ; Endothelin-1 ; Collagen gel ; Liver ; Rat (Wistar)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Hepatic stellate cells become activated and aquire contractility on being cultured. In order to characterize the morphology of contracted and relaxed stellate cells, we performed light- and electron-microscopic analyses of cultured stellate cells on collagen gels. Incubation of stellate cells with medium alone, 10 nM endothelin (ET)-1, or 1 mM N6,2′dibutyryladenosine 3′:5′-cyclic monophosphate (dBcAMP) for 48 h induced contraction of the underlying collagen gels to 83%, 57%, and 97%, respectively, of their original size. Stellate cells relaxed by dBcAMP exhibited a round cell body and extended several long thin cytoplasmic processes with several varicosities. Culture with ET-1 accelerated spreading of the stellate cells on collagen gels and decreased the number of processes. Each such flattened stellate cell attached itself to the underlying collagen matrix by bending its cell body. Collagen fibers around the cell were pulled toward the cell and stretched. Thus, the present study has revealed that ET-1-stimulated cultured stellate cells adduct associated collagen fibers by the retraction of cytoplasmic processes and the bending of their spread cell bodies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004410050717
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  • 9
    Electronic Resource
    Electronic Resource
    Three-dimensional structure of endothelial cells in hepatic sinusoids of the rat as revealed by the Golgi method (1988)
    Springer
    Cell & tissue research 253 (1988), S. 563-571 
    Details
    ISSN: 1432-0878
    Keywords: Endothelial cells ; Liver ; Sinusoids ; Golgi method ; 200 kV electron microscopy ; Rat (Wistar)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The three-dimensional structure of endothelial cells in the hepatic sinusoids of the rat was studied by application of light- and electron microscopy on Golgi-impregnated specimens. A number of endothelial cells could thus be individually delineated throughout the hepatic lobules. The cytoplasm, showing heavy silver deposits, consists of two distinct areas, a thick and thin portion. The thick portion, issuing from the region of the perikaryon, branches and tapers toward the cell periphery. The thin portion, occupying the remainder of the cytoplasm, consists largely of highly fenestrated sieve plates. Some intralobular variation can be noted; the thick portion of the endothelial cells is well developed in the periportal zone, while the cells in the centrilobular zone are relatively rich in thin portions. In addition, the area of distribution of an individual endothelial cell is larger in the centrilobular sinusoids than in the periportal zone. Some endothelial cells also possess unique cytoplasmic processes projecting into the intercellular space between hepatocytes and connecting the sinusoidal walls of neighboring sinusoids. These processes may anchor the endothelial cells to the hepatic plates.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00219747
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  • 10
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    Quantitative analysis of rod-cored vesicles and dense granules of large granular lymphocytes in the liver, spleen, and peripheral blood of rats (1994)
    Springer
    Details
    Publication Date: 1994-04-01
    Print ISSN: 0302-766X
    Electronic ISSN: 1432-0878
    Topics: Biology , Medicine
    Published by Springer
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