ISSN:
1432-1955
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract Trypanosomes, isolated from the gut of a naturally infectedGlossina pallidipes in Kiboko, Kenya, were grown in vitro. The cultured trypanosomes (“F4”-stock) showed a wide variety in morphological stages, not characteristic of the salivarian trypanosomes that are known to occur in the Kiboko area. Identification of the “F4”-stock was attempted by isoenzyme studies, infection of tsetse flies and of experimental animals. Electrophoretic isoenzyme patterns of the “F4”-organisms were developed for ten soluble enzymes and compared with those of 5 reference stocks of salivarian trypanosomes and 2 stocks ofTrypanosoma theileri. For theT. theileri comparisons six enzymes were used. It was found that the “F4”-trypanosome clearly differed from the 3 salivarian species and fromT. theileri. Experimental infection of laboratory bredGlossina m. morsitans showed that the “F4”-organisms developed in the posterior station of the alimentary tract and the cultured trypanosomes were infective to non-indigenous reptiles. This is believed to be the first record of the isolation and the isoenzyme characterisation of a non-salivarian reptile trypanosome from a naturally infected tsetse fly of themorsitans group (subgenusGlossina) in a savannah area in Africa.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00934006
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