Publication Date:
1982-09-10
Description:
The release of gamma-aminobutyric acid was confirmed in isolated cat colon loaded with tritiated gamma-aminobutyric acid. Thirty to 180 minutes after loading the spontaneous efflux of tritium appeared to fit a single exponential curve with an efflux rate coefficient of 0.002 per minute. Electrical stimulation produced frequency-dependent increases in the tritium efflux and in the contractions. Even 120 minutes later over 91 percent of the total radioactivity in the superfusates was attributable to tritiated gamma-aminobutyric acid. The acid release and the contractions induced by electrical transmural stimulation were inhibited by tetrodotoxin and by a calcium-free medium. Release of the acid was not significant during contractions elicited by nicotine and acetylcholine. These findings indicate that gamma-aminobutyric acid is released from the terminals of neurons in the myenteric plexus of the colon.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Taniyama, K -- Kusunoki, M -- Saito, N -- Tanaka, C -- New York, N.Y. -- Science. 1982 Sep 10;217(4564):1038-40.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7112110" target="_blank"〉PubMed〈/a〉
Keywords:
Acetylcholine/pharmacology
;
Animals
;
Calcium/physiology
;
Cations, Divalent/pharmacology
;
Cats
;
Colon/innervation/*metabolism
;
Electric Stimulation
;
Female
;
In Vitro Techniques
;
Male
;
Muscle Contraction
;
Muscle, Smooth
;
Nicotine/pharmacology
;
Tetrodotoxin/pharmacology
;
gamma-Aminobutyric Acid/*metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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