Publication Date:
2014-07-18
Description:
Corneal epithelial homeostasis and regeneration are sustained by limbal stem cells (LSCs), and LSC deficiency is a major cause of blindness worldwide. Transplantation is often the only therapeutic option available to patients with LSC deficiency. However, while transplant success depends foremost on LSC frequency within grafts, a gene allowing for prospective LSC enrichment has not been identified so far. Here we show that ATP-binding cassette, sub-family B, member 5 (ABCB5) marks LSCs and is required for LSC maintenance, corneal development and repair. Furthermore, we demonstrate that prospectively isolated human or murine ABCB5-positive LSCs possess the exclusive capacity to fully restore the cornea upon grafting to LSC-deficient mice in xenogeneic or syngeneic transplantation models. ABCB5 is preferentially expressed on label-retaining LSCs in mice and p63alpha-positive LSCs in humans. Consistent with these findings, ABCB5-positive LSC frequency is reduced in LSC-deficient patients. Abcb5 loss of function in Abcb5 knockout mice causes depletion of quiescent LSCs due to enhanced proliferation and apoptosis, and results in defective corneal differentiation and wound healing. Our results from gene knockout studies, LSC tracing and transplantation models, as well as phenotypic and functional analyses of human biopsy specimens, provide converging lines of evidence that ABCB5 identifies mammalian LSCs. Identification and prospective isolation of molecularly defined LSCs with essential functions in corneal development and repair has important implications for the treatment of corneal disease, particularly corneal blindness due to LSC deficiency.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4246512/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4246512/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Ksander, Bruce R -- Kolovou, Paraskevi E -- Wilson, Brian J -- Saab, Karim R -- Guo, Qin -- Ma, Jie -- McGuire, Sean P -- Gregory, Meredith S -- Vincent, William J B -- Perez, Victor L -- Cruz-Guilloty, Fernando -- Kao, Winston W Y -- Call, Mindy K -- Tucker, Budd A -- Zhan, Qian -- Murphy, George F -- Lathrop, Kira L -- Alt, Clemens -- Mortensen, Luke J -- Lin, Charles P -- Zieske, James D -- Frank, Markus H -- Frank, Natasha Y -- DP2 OD007483/OD/NIH HHS/ -- DP2OD007483/OD/NIH HHS/ -- EY08098/EY/NEI NIH HHS/ -- I01 BX000516/BX/BLRD VA/ -- I01 RX000989/RX/RRD VA/ -- K08 NS051349/NS/NINDS NIH HHS/ -- K08NS051349/NS/NINDS NIH HHS/ -- P30 EY014801/EY/NEI NIH HHS/ -- P30EY014801/EY/NEI NIH HHS/ -- P41EB015903/EB/NIBIB NIH HHS/ -- R01 CA113796/CA/NCI NIH HHS/ -- R01 CA138231/CA/NCI NIH HHS/ -- R01 CA158467/CA/NCI NIH HHS/ -- R01 EB017274/EB/NIBIB NIH HHS/ -- R01CA113796/CA/NCI NIH HHS/ -- R01CA138231/CA/NCI NIH HHS/ -- R01CA158467/CA/NCI NIH HHS/ -- R01EY018624/EY/NEI NIH HHS/ -- R01EY021768/EY/NEI NIH HHS/ -- U01HL100402/HL/NHLBI NIH HHS/ -- Howard Hughes Medical Institute/ -- England -- Nature. 2014 Jul 17;511(7509):353-7. doi: 10.1038/nature13426. Epub 2014 Jul 2.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉1] Department of Ophthalmology, Schepens Eye Research Institute, Massachusetts Eye & Ear Infirmary and Harvard Medical School, Boston, Massachusetts 02114, USA [2]. ; 1] Transplant Research Program, Division of Nephrology, Boston Children's Hospital, Boston, Massachusetts 02115, USA [2] Department of Dermatology, Brigham and Women's Hospital, Boston, Massachusetts 02115, USA [3] Department of Medicine, VA Boston Healthcare System, Boston, Massachusetts 02130, USA. ; 1] Transplant Research Program, Division of Nephrology, Boston Children's Hospital, Boston, Massachusetts 02115, USA [2] Department of Dermatology, Brigham and Women's Hospital, Boston, Massachusetts 02115, USA. ; 1] Department of Medicine, VA Boston Healthcare System, Boston, Massachusetts 02130, USA [2] Transplant Research Program, Division of Nephrology, Boston Children's Hospital, Boston, Massachusetts 02115, USA [3] Department of Dermatology, Brigham and Women's Hospital, Boston, Massachusetts 02115, USA. ; Department of Ophthalmology, Schepens Eye Research Institute, Massachusetts Eye & Ear Infirmary and Harvard Medical School, Boston, Massachusetts 02114, USA. ; Bascom Palmer Eye Institute and the Department of Ophthalmology, University of Miami Miller School of Medicine, Miami, Florida 33136, USA. ; Department of Ophthalmology, University of Cincinnati Medical Center, Cincinnati, Ohio 45229, USA. ; Stephen A Wynn Institute for Vision Research, Carver College of Medicine, Department of Ophthalmology and Visual Sciences, University of Iowa, Iowa City, Iowa 52242, USA. ; Department of Pathology, Brigham and Women's Hospital, Boston, Massachusetts 02115, USA. ; Department of Ophthalmology, University of Pittsburgh School of Medicine & Department of Bioengineering, University of Pittsburgh Swanson School of Engineering, Pittsburgh, Pennsylvania 15213, USA. ; Center for Systems Biology and Wellman Center for Photomedicine, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114, USA. ; 1] Transplant Research Program, Division of Nephrology, Boston Children's Hospital, Boston, Massachusetts 02115, USA [2] Department of Dermatology, Brigham and Women's Hospital, Boston, Massachusetts 02115, USA [3] Harvard Stem Cell Institute, Harvard Medical School, Boston, Massachusetts 02138, USA [4]. ; 1] Department of Medicine, VA Boston Healthcare System, Boston, Massachusetts 02130, USA [2] Transplant Research Program, Division of Nephrology, Boston Children's Hospital, Boston, Massachusetts 02115, USA [3] Harvard Stem Cell Institute, Harvard Medical School, Boston, Massachusetts 02138, USA [4] Division of Genetics, Brigham and Women's Hospital, Boston, Massachusetts 02115, USA [5].〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/25030174" target="_blank"〉PubMed〈/a〉
Keywords:
ATP-Binding Cassette Transporters/deficiency/*metabolism
;
Animals
;
Apoptosis
;
Biomarkers/metabolism
;
Cell Differentiation
;
Cell Proliferation
;
Female
;
Humans
;
Limbus Corneae/*cytology/*physiology
;
Male
;
Mice
;
Mice, Knockout
;
Molecular Sequence Data
;
P-Glycoprotein/deficiency/*metabolism
;
*Regeneration
;
Stem Cell Transplantation
;
Stem Cells/cytology/*metabolism
;
Transcription Factors/metabolism
;
Tumor Suppressor Proteins/metabolism
;
*Wound Healing
Print ISSN:
0028-0836
Electronic ISSN:
1476-4687
Topics:
Biology
,
Chemistry and Pharmacology
,
Medicine
,
Natural Sciences in General
,
Physics
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