Publication Date:
1987-10-23
Description:
X-ray holography offers the possibility of three-dimensional microscopy with resolution higher than that of the light microscope and with contrast based on x-ray edges. In principle, the method is especially advantageous for biological samples if x-rays in the wavelength region between the carbon and oxygen K edges are used. However, until now the achieved resolution has not exceeded that of the light microscope because of the poor coherence properties of the x-ray sources and the low resolution of the detectors that were available. With a recently developed x-ray source based on an undulator on an electron storage ring, and high resolution x-ray resist, a hologram has been recorded at about 400-angstrom resolution. The experiment utilized x-rays with wavelengths of 24.7 angstroms and required a 1-hour exposure of the pancreatic zymogen granules under study.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Howells, M -- Jacobsen, C -- Kirz, J -- Feder, R -- McQuaid, K -- Rothman, S -- New York, N.Y. -- Science. 1987 Oct 23;238(4826):514-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Center for X-ray Optics, Lawrence Berkeley Laboratory, University of California, Berkeley 94720.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3659925" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Cytoplasmic Granules/enzymology/*ultrastructure
;
*Enzyme Precursors
;
Holography/*methods
;
Microscopy, Electron
;
Pancreas/ultrastructure
;
Rats
;
X-Rays
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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