Publication Date:
1998-02-07
Description:
Dynamin guanosine triphosphatases support the scission of clathrin-coated vesicles from the plasmalemma during endocytosis. By fluorescence microscopy of cultured rat hepatocytes, a green fluorescent protein-dynamin II fusion protein localized with clathrin-coated vesicles at the Golgi complex. A cell-free assay was utilized to demonstrate the role of dynamin in vesicle formation at the trans-Golgi. Addition of peptide-specific anti-dynamin antibodies to the assay mixture inhibited both constitutive exocytic and clathrin-coated vesicle formation. Immunodepletion of dynamin proteins also inhibited vesicle formation, and budding efficiency was restored upon readdition of purified dynamin. These data suggest that dynamin participates in the formation of distinct transport vesicles from the trans-Golgi network.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Jones, S M -- Howell, K E -- Henley, J R -- Cao, H -- McNiven, M A -- P30 CA-46934/CA/NCI NIH HHS/ -- P30 DK-34914/DK/NIDDK NIH HHS/ -- New York, N.Y. -- Science. 1998 Jan 23;279(5350):573-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Center for Basic Research in Digestive Diseases, Mayo Clinic and Foundation, Rochester, MN 55905, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/9438853" target="_blank"〉PubMed〈/a〉
Keywords:
Adenosine Triphosphate/metabolism
;
Animals
;
Biological Transport
;
Cell Membrane/chemistry
;
Cells, Cultured
;
Clathrin/analysis
;
Coated Vesicles/chemistry/*metabolism/ultrastructure
;
Cytosol/metabolism
;
Dynamins
;
GTP Phosphohydrolases/analysis/immunology/*metabolism
;
Golgi Apparatus/chemistry/*metabolism
;
Green Fluorescent Proteins
;
Guanosine 5'-O-(3-Thiotriphosphate)/metabolism
;
Liver/ultrastructure
;
Luminescent Proteins
;
Microscopy, Fluorescence
;
Organelles/chemistry/*metabolism/ultrastructure
;
Rats
;
Recombinant Fusion Proteins/analysis
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
Permalink