Publication Date:
2004-01-13
Description:
The lasting effects of neuronal activity on brain development involve calcium-dependent gene expression. Using a strategy called transactivator trap, we cloned a calcium-responsive transactivator called CREST (for calcium-responsive transactivator). CREST is a SYT-related nuclear protein that interacts with adenosine 3',5'-monophosphate (cAMP) response element-binding protein (CREB)-binding protein (CBP) and is expressed in the developing brain. Mice that have a targeted disruption of the crest gene are viable but display defects in cortical and hippocampal dendrite development. Cortical neurons from crest mutant mice are compromised in calcium-dependent dendritic growth. Thus, calcium activation of CREST-mediated transcription helps regulate neuronal morphogenesis.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Aizawa, Hiroyuki -- Hu, Shu-Ching -- Bobb, Kathryn -- Balakrishnan, Karthik -- Ince, Gulayse -- Gurevich, Inga -- Cowan, Mitra -- Ghosh, Anirvan -- MH60598/MH/NIMH NIH HHS/ -- NS39993/NS/NINDS NIH HHS/ -- New York, N.Y. -- Science. 2004 Jan 9;303(5655):197-202.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/14716005" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Sequence
;
Animals
;
Blotting, Northern
;
Brain/cytology/embryology/growth & development/metabolism
;
CREB-Binding Protein
;
Calcium/*metabolism
;
Calcium Channels/metabolism
;
Cell Line
;
Cells, Cultured
;
Cerebral Cortex/cytology/embryology/metabolism
;
Cloning, Molecular
;
Dendrites/*physiology/ultrastructure
;
Gene Expression Profiling
;
Gene Expression Regulation, Developmental
;
Gene Library
;
Gene Targeting
;
Humans
;
In Situ Hybridization
;
Mice
;
Mice, Knockout
;
Molecular Sequence Data
;
Mutation
;
Nervous System/embryology/growth & development/metabolism
;
Neurons/*physiology/ultrastructure
;
Nuclear Proteins/metabolism
;
Protein Structure, Tertiary
;
Rats
;
Recombinant Fusion Proteins/metabolism
;
Trans-Activators/chemistry/genetics/*metabolism
;
*Transcription, Genetic
;
*Transcriptional Activation
;
Transfection
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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