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  • 1
    ISSN: 1432-072X
    Keywords: Methanobacterium thermoautotrophicum ; Bacteriophage ; Methanogenic bacteria ; Plasmid pME2001
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Bacteriophage ψM1, a virulent, oxygen resistant phage of Methanobacterium thermoautotrophicum strain Marburg, was isolated from an anaerobic sludge digester operated at 55°C to 60°C. A reproducible plaque assay and an enrichment procedure for the preparation of high-titer lysates (2x1010 PFU/ml) were established. One-step growth experiments at 62°C showed that the latent period was 4 h and the burst size was 5–6 infective particles per cell. The phage infected Methanobacterium thermoautotrophicum Marburg but none of three other thermophilic representatives of the genus Methanobacterium that were tested. Electron micrographs showed that phage ψM1 has a polyhedral head of 55 nm diameter and a tail of 210 nm in lenght. The ψM1 genome consists of linear double-stranded DNA with a size of 30.4±1.0 kb. Restriction and hybridization analysis of DNA extracted from phage particles revealed two types of linear molecules with the size of the phage genome. About 85% of the DNA molecules in such preparations were genomes of ψM1 whereas approximately 15% were multimers of the cryptic 4.5-kb plasmid pME2001 of the host. ψM1 DNA did not hybridize with chromosomal DNA of Methanobacterium thermoautotrophicum but it exhibited definite homology to total DNA of Methanobacterium wolfei.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-0778
    Keywords: calcium phosphate ; transfection ; transient expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract This is the first report of transient transfection of suspended cells with purified plasmid DNA in bioreactors or spinner flasks. DNA/calcium phosphate complexes were pumped or injected directly into stirred cultures of the immortalized human embryo kidney cell line 293 (HEK-293) which had been adapted to growth in suspension. We identified culture conditions suitable for this approach and modified the protocol for the generation of precipitate complexes, based on our earlier work. In order to stabilize the ‘DNA-vehicle’-complex in the culture medium, we identified pH ranges and ion-concentrations which prevent dissolution or aggregation of the precipitate particles. Such conditions maintained suspended fine particles in spinners or bioreactors for up to 6 hr. During that period, cells and precipitate complexes interacted sufficiently to allow DNA transfer and subsequent expression of recombinant protein. In a simple 5 day batch process, with a starting density of 0.3 × 106 cells mL-1, about 0.5 mg L-1 of a recombinant tissue plasminogen activator variant was observed.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 220 (1989), S. 161-164 
    ISSN: 1617-4623
    Keywords: Methanogenic bacteria ; Methanobacterium thermoautotrophicum ; Bacteriophage ψ M1 ; Bacteriophage genome ; Packaging
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary ψ M1 is a virulent bacteriophage of Methanobacterium thermoautotrophicum strain Marburg. Restriction enzyme analysis of the linear, 30.4 kb phage DNA led to a circular map of the 27.1 kb ψ M1 genome. ψ M1 is thus circularly permuted and exhibits terminal redundancy of approximately 3 kb. Packaging of ψ M1 DNA from a concatemeric precursor initiates at the pac site which was identified at coordinate 4.6 kb on the circular genome map. It proceeds clockwise for at least five packaging rounds. Headful packaging was also shown for ψ M2, a phage variant with a 0.7 kb deletion at coordinate 23.25 on the map.
    Type of Medium: Electronic Resource
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  • 4
    Publication Date: 2012-06-14
    Description: In the context of global warming, hydrological regimes in mountain areas are likely to be modified and will therefore result in significant impacts to the supply mechanisms for hydropower and other end-uses of water, both in the mountains themselves and in the lowland regions downstream. The main objective here is to attempt a fine and continuous analysis of the impacts of such changes on runoff and glaciers in a largely ice-covered catchment in the Swiss Alps: the Findelen watershed. The simulated daily discharge values have been obtained with a semi-distributed hydrological model called Routing System 3 (RS3.0). A stochastic weather generator has been used to generate a 110 year sequence of meteorological input data—described in a companion article—that have been further perturbed in order to simulate a progressively warmer climate by the end of the century. The amplitude of atmospheric change is suggested by regional climate model simulations, under the A2 greenhouse-gas emissions scenario. Results show at first an increase of discharge (19.4% in about 60 years) due to the rapid melt of the glacier, followed by a large decrease in runoff at the end of the period (−28% from the beginning to the end of the studied timeframe), primarily due to the depletion of the solid water reservoir. The glacier is indeed projected to lose 91% of its surface area. In parallel, a seasonal shift in flow patterns is also observed: the discharge values are high earlier in spring due to advanced snow and ice melt, while the rest of the curve flattens out. Copyright © 2012 Royal Meteorological Society
    Print ISSN: 0899-8418
    Electronic ISSN: 1097-0088
    Topics: Geosciences , Physics
    Published by Wiley
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  • 5
    Publication Date: 2012-05-18
    Description: The complex topography of the Alps makes detailed hydrological modelling a real challenge. It is yet an essential task to improve the insight of hydrological processes in the context of intensification of renewable energy use and under the constraints of climate change. In this perspective and as a case study, the runoff of a small highly glacierized basin in the Swiss Alps—namely the Findelen catchment area—has been modelled with a hydrological model (Routing System 3.0; RS3.0). It is a conceptual model, based on object-oriented programming and it computes snow-melt, glaciers, infiltration and runoff processes. As input, it requires hourly air temperatures and precipitation, and the geomorphologic features of the catchment area and glacier. RS3.0 has proven to be very efficient in reproducing discharge. To evaluate the impacts of climate change on runoff—the final objective of the study addressed in a companion paper—a stochastic meteorological data generator has been developed to reproduce a sequence of air temperature and precipitation over more than one century. In this way, a continuous series of daily discharge values has been simulated by RS3.0 with the stochastic input data. This methodology has also enabled an assessment of the glacier response time to a stable climate. Indeed, if the climatic conditions of the standard reference period 1961-1990 were to be preserved throughout the 21st century, the simulation shows that the watershed would be slow to adapt: the glaciers would be balanced with the atmospheric conditions and the water discharge would reach a lower stable value in more than 40 years. However, the glacierized area would lose only 3.5% of its surface. Copyright © 2012 Royal Meteorological Society
    Print ISSN: 0899-8418
    Electronic ISSN: 1097-0088
    Topics: Geosciences , Physics
    Published by Wiley
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  • 6
    Publication Date: 2018-03-30
    Description: Sustainability, Vol. 10, Pages 1011: Evolution of Rural Livelihood Strategies in a Remote Sino-Mongolian Border Area: A Cross-Country Analysis Sustainability doi: 10.3390/su10041011 Authors: Munkhnasan Tsvegemed Alimu Shabier Eva Schlecht Greta Jordan Martin Wiehle Ecologically sound natural resources management is still the backbone of rural livelihoods in many regions of the world. The Altai-Dzungarian region between China and Mongolia constitutes an ideal site to study how political, economic, infrastructural, and cultural differences affect rural livelihoods. Structured semi-quantitative interviews were conducted with 483 households on both sides to characterise their current livelihood strategies and assess the importance of the various activities for the households’ current socio-economic situation by means of the categorical principal component and two-step cluster analysis. In total, four livelihood clusters were identified across both regions, whereby one cluster was only present in Mongolia. In general, all clusters mirrored the transition from almost pure pastoralist to agro-pastoralist livelihood strategies. While animal husbandry was more common in Mongolia and crop farming more common in China, most households in both countries pursued a rather mixed approach. The composition of the herds, as well as the richness and diversity of the livestock species, differed significantly between the countries and was generally higher in Mongolia. Supplementary feedstuff and pesticide and fertiliser use were higher in China, along with diversification of produces. Our analysis indicates that until very recently the livelihood strategies on both sides of the border were the same, manifesting in the fact that we can define three identical clusters across countries (environment factor) even though there are slight differences in land, livestock and asset endowment.
    Electronic ISSN: 2071-1050
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Published by MDPI Publishing
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  • 7
    Publication Date: 1989-07-01
    Print ISSN: 0302-8933
    Electronic ISSN: 1432-072X
    Topics: Biology
    Published by Springer
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  • 8
    Publication Date: 2018-03-21
    Description: Genes, Vol. 9, Pages 171: Genomic Characterization of Listeria monocytogenes Isolates Associated with Clinical Listeriosis and the Food Production Environment in Ireland Genes doi: 10.3390/genes9030171 Authors: Amber Hilliard Dara Leong Amy O’Callaghan Eamonn Culligan Ciara Morgan Niall DeLappe Colin Hill Kieran Jordan Martin Cormican Cormac Gahan Listeria monocytogenes is a major human foodborne pathogen that is prevalent in the natural environment and has a high case fatality rate. Whole genome sequencing (WGS) analysis has emerged as a valuable methodology for the classification of L. monocytogenes isolates and the identification of virulence islands that may influence infectivity. In this study, WGS was used to provide an insight into 25 L. monocytogenes isolates from cases of clinical infection in Ireland between 2013 and 2015. Clinical strains were either lineage I (14 isolates) or lineage II (11 isolates), with 12 clonal complexes (CC) represented, of which CC1 (6) and CC101 (4) were the most common. Single nucleotide polymorphism (SNP) analysis demonstrated that clinical isolates from mother–infant pairs (one isolate from the mother and one from the infant) were highly related (3 SNP differences in each) and also identified close similarities between isolates from otherwise distinct cases (1 SNP difference). Clinical strains were positive for common virulence-associated loci and 13 isolates harbour the LIPI-3 locus. Pulsed-field gel electrophoresis (PFGE) was used to compare strains to a database of 1300 Irish food and food processing environment isolates and determined that 64% of clinical pulsotypes were previously encountered in the food or food processing environment. Five of the matching food and food processing environment isolates were sequenced and results demonstrated a correlation between pulsotype and genotype. Overall, the work provides insights into the nature of L. monocytogenes strains currently causing clinical disease in Ireland and indicates that similar isolates can be found in the food or food processing environment.
    Electronic ISSN: 2073-4425
    Topics: Biology
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  • 9
    Publication Date: 2012-06-14
    Print ISSN: 0920-9069
    Electronic ISSN: 1573-0778
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Published by Springer
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  • 10
    Publication Date: 2019-04-26
    Description: (1) Background: Bone substitutes are essential in orthopaedic surgery to fill up large bone defects. Thus, the aim of the study was to compare diverse bone fillers biomechanically to each other in a clinical-relevant test set-up and to detect differences in stability and handling for clinical use. (2) Methods: This study combined compressive strength tests and screw pullout-tests with dynamic tests of bone substitutes in a clinical-relevant biomechanical fracture model. Beyond well-established bone fillers (ChronOSTM Inject and Graftys® Quickset), two newly designed bone substitutes, a magnesium phosphate cement (MPC) and a drillable hydrogel reinforced calcium phosphate cement (CPC), were investigated. (3) Results: The drillable CPC revealed a comparable displacement of the fracture and maximum load to its commercial counterpart (Graftys® Quickset) in the clinically relevant biomechanical model, even though compressive strength and screw pullout force were higher using Graftys®. (4) Conclusions: The in-house-prepared cement allowed unproblematic drilling after replenishment without a negative influence on the stability. A new, promising bone substitute is the MPC, which showed the best overall results of all four cement types in the pure material tests (highest compressive strength and screw pullout force) as well as in the clinically relevant fracture model (lowest displacement and highest maximum load). The low viscosity enabled a very effective interdigitation to the spongiosa and a complete filling up of the defect, resulting in this demonstrated high stability. In conclusion, the two in-house-developed bone fillers revealed overall good results and are budding new developments for clinical use.
    Electronic ISSN: 1996-1944
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
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