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  • 1
    ISSN: 1432-2242
    Keywords: Key words Microspore culture  ;  Quantitative trait locus (QTL)  ;  Multiple QTL models (MQM)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A genetic map covering 615 cM in 12 linkage groups was assembled based on 92 RFLP and AFLP markers segregating in a population of 107 doubled haploid lines (DH lines) of Brassica oleracea. The DH-line population was obtained through microspore culture from the of two homozygous parents: DH-line Bi derived from the cabbage landrace Bindsachsener, and DH-line Gr from broccoli cv ‘Greenia’. Sixty-five percent of the loci, and in some cases complete linkage groups, displayed distorted segregation ratios, a frequency much higher than that observed in populations of the same species. DH-line Bi was resistant to clubroot, which is caused by a Dutch field isolate of Plasmodiophora brassicae. Resistance in the DH-line population was determined in two ways: by assigning symptom grades to each plant, and by measuring the fresh weights of the healthy and affected parts of the root system of each plant. Using a multiple QTL mapping approach to analyze the fresh weight data, we found two loci for clubroot resistance; these were designated pb-3 and pb-4. The additive effects of these loci were responsible for 68% of the difference between the parents and for 60% of the genetic variance among DH-line means. Also, indications for the presence of two additional, minor QTLs were found. Analysis of symptom grades revealed the two QTLs pb-3 and pb-4, as well as one of the two minor QTLs indicated by analysis of the fresh weight data.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Plant systematics and evolution 163 (1989), S. 133-146 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Cucurbitaceae ; Cucumis ; C. sativus ; C. melo ; C. metuliferus ; C. anguria ; C. zeyheri ; C. myriocarpus subsp.leptodermis ; comb. nov. ; Crossing experiments ; meiosis of hybrids ; polyploidy ; isozymes ; DNA analysis ; evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Meiosis in seven interspecificCucumis hybrids has been analysed i.a. inC. metuliferus ×C. zeyheri, where the parents belong to different sections. In the triploid hybrids a remarkably high number of trivalents has been found. Additional data from literature on geographical distribution, cucurbitacins, flavonoid patterns, isozymes, C-banding, genome size, DNA amount and chloroplast DNA are used to discuss species relationships and evolution. The African cross-compatible group is divided into theMyriocarpus subgroup with the diploid speciesC. africanus, C. myriocarpus subsp.leptodermis and subsp.myriocarpus, and theAnguria subgroup withC. anguria, C. dipsaceus, C. ficifolius, C. prophetarum, C. zeyheri and all polyploids (exceptC. heptadactylus). It is argued that the Asian subg.Melo with x = 7 is derived from the African subg.Cucumis with x = 12; the latter contains all the polyploid species and has the most common basic chromosome number of theCucurbitaceae. This phylogenetic advance is interpreted with concepts of the quantum model of evolution.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Euphytica 35 (1986), S. 73-76 
    ISSN: 1573-5060
    Keywords: Tulipa ; tulip ; species ; diploid ; polyploid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Chromosome numbers of Tulipa species were determined. For the first time hexaploidy was found in the genus. It became clear that in many species or subspecies several ploidy levels may occur.
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  • 4
    ISSN: 1573-5060
    Keywords: Allium cepa ; Allium roylei ; disease resistance ; interspecific crosses ; linkage groups ; onion breeding ; taxonomic relationships
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Male meiosis of the interspecific hybrid between Allium roylei and A. cepa is undisturbed relative to its parents. Based on meiotic data, A. roylei is concluded to be a closer relative of A. cepa than A. fistulosum. Segregation ratios for downy mildew resistance among BC1 and F2 progenies from the F1 between A. roylei and A. cepa indicate the presence of two dominantly inherited, weakly linked nuclear resistance genes, Pd 1 and Pd 2, in A. roylei (recombination frequency 0.32±0.03). Presumably Pd 1 and Pd 2are the first genes described in Allium residing in one linkage group. The prospects of exploiting A. roylei in onion breeding seem very promising.
    Type of Medium: Electronic Resource
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