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  • 1
    Electronic Resource
    Electronic Resource
    Chemiluminescence response and adherence of neutrophils to cultured endothelial cells-influence of immunoglobulin G (1995)
    New York : Wiley-Blackwell
    Journal of Bioluminescence and Chemiluminescence 10 (1995), S. 169-173 
    Details
    ISSN: 0884-3996
    Keywords: Immunoglobulin G ; endothelium ; polymorphonuclear leukocyte ; cell interaction ; adherence ; oxygen derived metabolites ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: The effect of different concentrations (0.87, 4.35, 8.7, 17.5, 25 and 35 mg/mL) of intravenous immunoglobulin G (Endobulin®) on neutrophil-endothelial cell interaction was studied using an in vitro model of human umbilical cord vein endothelial cells and human neutrophils. Because adherence of neutrophils to endothelial cells is an essential component in inflammatory processes leading to endothelial cell injury the influence of immunoglobulin G on adherence has been investigated.A second aim of the present study was to determine changes in chemiluminescence response of neutrophils during adherence to endothelial cells. Production of oxygen-derived metabolites, measured by chemiluminescence response of neutrophils, decreased significantly in the presence of 8.7 mg immunoglobulin/mL test during coincubation of neutrophils and endothelial cells (p 〈 0.025). The adherence of neutrophils to endothelial cells was significantly decreased at a concentration of 8.7 mg immunoglobulin/mL test (p 〈 0.025).The present results indicate that this preparation of immunoglobulin G might exert a protective effect on neutrophil-endothelial cell interaction by decreasing adherence of neutrophils to endothelial cells and by scavenging reactive oxygen metabolites. Therefore, the current investigation points to a probable protective effect of immunoglobulin G in oxidative diseases, such as the adult respiratory distress syndrome.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bio.1170100305
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Effect of prostaglandin E1 on chemiluminescence response and adherence of human polymorphonuclear leukocytes to human cultured endothelial cells of prostaglandin E1 treated polytraumatized patients (1993)
    New York : Wiley-Blackwell
    Journal of Bioluminescence and Chemiluminescence 8 (1993), S. 253-260 
    Details
    ISSN: 0884-3996
    Keywords: Prostaglandin E1 ; endothelium ; polymorphonuclear leukocyte ; cell interaction ; adherence ; oxygen-derived metabolites ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: We investigated the effect of prostaglandin E1 on human polymorphonuclear leukocytes, in vivo. Polymorphonuclear leukocytes of a prostaglandin E1 and placebo study group were harvested and their function, as production of oxygen-derived metabolites and adherence to human cultured endothelial cells, was compared. Additionally, data obtained from polymorphonuclear leukocytes of a prostaglandin E1 and placebo group were compared with data obtained from polymorphonuclear leukocytes from 28 blood donors, who served as a control group.Production of oxygen-derived metabolites by polymorphonuclear leukocytes during contact with endothelial cells was measured by chemiluminescence. Chemiluminescence was significantly (p 〈 0.01) increased in the placebo group in comparison to the control group decreasing to values of control group after 6 d (post-trauma). Chemiluminescence response was not significantly suppressed in patients treated with prostaglandin E1 in comparison to the placebo group. Adherence of polymorphonuclear leukocytes (placebo group) to endothelial cells was significantly increased (p 〈 0.01) within the first 6 d post-trauma Following day 6, values were in the same range as values for the control group. Adherence was not significantly suppressed in patients treated with prostaglandin E1 in comparison to the placebo group. In conclusion, prostaglandin E1 at a dose of 20 ng/kg bw/min does not influence production of oxygenderived metabolites and adherence in polytraumatized patients in comparison to a placebo group. Additionally, production of oxygen-derived metabolites by polymorphonuclear leukocytes in response to endothelial cells is shown and it is evident that endothelial cells might influence production of oxygen derived metabolites by polymorphonuclear leukocytes.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bio.1170080505
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  • 3
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    Schauplätze des Reparierens und Selbermachens (2022)
    transcript Verlag | transcript Verlag
    Details
    Publication Date: 2022-05-12
    Description: Können die Schauplätze des Repair- und Do-it-yourself-Urbanismus wesentliche Beiträge zu einer nachhaltigen Stadtentwicklung leisten, die die hegemonialen Praktiken heutiger Wegwerfgesellschaften überwinden? Anhand einer praxeologischen Forschung in zwei Wiener Bezirken analysieren die Autor*innen die Schauplätze des Tauschens und Teilens, des Reparierens, des Upcyclings und des Kreativ-Seins, in denen sich Menschen im öffentlichen Raum für den Erhalt von Dingen engagieren. Mit dem Begriff des Schauplatzes rücken sie die öffentlichkeitswirksamen Praktiken eines nachhaltigen Umgangs mit den Dingen in den Vordergrund und verbinden diese mit aktuellen Diskussionen über notwendige Entwicklungen urbaner Infrastrukturen der Sorge und der Suffizienz.
    Keywords: Reparieren ; Selbermachen ; Praxis ; Infrastruktur ; Schauplatz ; Sorge ; Suffizienz ; DIY ; Tausch ; Upcycling ; Stadt ; Gesellschaft ; Wirtschaft ; Urban Studies ; Nachhaltigkeit ; Zivilgesellschaft ; Wirtschaftssoziologie ; Soziologie ; Repair Movement ; Do-it-yourself ; Practice ; Infrastructure ; Location ; Care ; Sufficiently ; Exchange ; City ; Society ; Economy ; Sustainability ; Civil Society ; Economic Sociology ; Sociology ; bic Book Industry Communication::J Society & social sciences::JF Society & culture: general::JFS Social groups::JFSG Urban communities ; bic Book Industry Communication::K Economics, finance, business & management::KC Economics::KCN Environmental economics ; bic Book Industry Communication::R Earth sciences, geography, environment, planning::RN The environment::RNU Sustainability ; bic Book Industry Communication::J Society & social sciences::JP Politics & government::JPW Political activism
    Language: German
    Format: image/jpeg
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    E-BOOK
  • 4
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    Low surface damage dry etched black silicon (2017)
    American Institute of Physics
    Details
    Publication Date: 2017-10-14
    Print ISSN: 0021-8979
    Electronic ISSN: 1089-7550
    Topics: Physics
    Published by American Institute of Physics
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    PAPER CURRENT
  • 5
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    Inducible gene modification in the gastric epithelium of Tff1-CreERT2;Tff2-rtTA;Tff3-Luc mice (2016)
    Wiley
    In: Genesis
    Details
    Publication Date: 2016-10-13
    Description: Temporal and spatial regulation of genes mediated by tissue-specific promoters and conditional gene expression systems provide a powerful tool to study gene function in health, disease and during development. Although transgenic mice expressing the Cre recombinase in the gastric epithelium have been reported, there is a lack of models that allow inducible and reversible gene modification in the stomach. Here, we exploited the gastrointestinal epithelium-specific expression pattern of the three trefoil factor ( Tff ) genes and bacterial artificial chromosome transgenesis to generate a novel mouse strain that expresses the CreERT2 recombinase and the reverse tetracycline transactivator (rtTA). The Tg( Tff1-CreERT2 ; Tff2-rtTA;Tff3-Luc ) strain confers tamoxifen-inducible irreversible somatic recombination and allows simultaneous doxycycline-dependent reversible gene activation in the gastric epithelium of developing and adult mice. This strain also confers luciferase activity to the intestinal epithelium to enable in vivo bioluminescence imaging. Using fluorescent reporters as conditional alleles, we show Tff1-CreERT2 and Tff2-rtTA transgene activity in a partially overlapping subset of long-term regenerating gastric stem/progenitor cells. Therefore, the Tg( Tff1-CreERT2 ; Tff2-rtTA; Tff3-Luc ) strain can confer intermittent transgene expression to gastric epithelial cells that have undergone previous gene modification, and may be suitable to genetically model therapeutic intervention during development, tumorigenesis and other genetically tractable diseases. This article is protected by copyright. All rights reserved.
    Topics: Biology , Medicine
    Published by Wiley
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  • 6
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    Deletion of the Mir-106b~ 25 MicroRNA cluster attenuates atherosclerosis in Apolipoprotein E knockout mice (2019)
    BioMed Central
    Details
    Publication Date: 2019-12-01
    Electronic ISSN: 1476-511X
    Topics: Biology
    Published by BioMed Central
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  • 7
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    Differential systemic inflammatory responses after TAVI: The role of self versus balloon expandable devices (2021)
    Public Library of Science
    Details
    Publication Date: 2021-10-26
    Description: Objective Transcatheter aortic valve implantation (TAVI) provokes early injury response, represented in part by dynamic changes in the inflammatory markers. The association of self-expanding valves (SEVs) and balloon-expandable valves (BEVs) with the consequent inflammatory response remains uncertain. Materials and methods Patients with severe symptomatic aortic stenosis who underwent transfemoral TAVI: SEVs or BEVs, from January 2010 to December 2019 were enrolled. Whole white blood cells (WBC) and subpopulation dynamics as well the neutrophil to lymphocyte ratio (NLR) were evaluated. Results Three-hundred seventy consecutive patients (mean age 81.75 ± 6.8 years, 199 women’s) were enrolled. In the entire population, significant kinetic changes in the WBC response (p
    Electronic ISSN: 1932-6203
    Topics: Medicine , Natural Sciences in General
    Published by Public Library of Science
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