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Distribution of Bs1 retrotransposons in Zea and related genera (1990)

Fuerstenberg, S. I. ; Johns, M. A.

Springer

Theoretical and applied genetics 80 (1990), S. 680-686

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ISSN: 1432-2242

Keywords: Retrotransposon ; Zea ; RFLP ; Bs1 ; Transposable element

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Summary Thirty-eight accessions from Zea and 20 accessions from related genera were probed for the presence of Bs1, a retrotransposon originally found in maize. All maize and teosinte plants tested show the presence of Bs1 in one to five densely hybridizing bands. The mean copy numbers of Bs1 elements among the maize and teosinte accessions were similar: 2.92 and 3.25, respectively, with no large differences between any subgroups. Most exotic maize samples exhibited two common bands of 7.8 kb and 4.7 kb. Section Zea teosintes (but not teosintes of section Luxuriantes) also show the presence of a common band of the same size as the smaller common band in maize. At reduced stringency, Tripsacum dactyloides exhibited a single hybridizing band at 6.9 kb. Results argue for the evolution of maize from a mexicana or parviglumis teosinte, and the evolution of the Bs1 element within the tribe Andropogoneae. Additionally, recombinant inbred lines were probed for the presence of Bs1, in order to map the chromosomal locations of Bs1 elements in four different maize lines. Two of the recombinant inbred parental lines had an element (Bs1-F) on chromosome 5, while the other two lines had an element (Bs1-S) on chromosome 8. Restriction site polymorphisms have apparently arisen in the vicinity of Bs1-S since its insertion. Segregation analysis of other lines was also performed; the data indicate that Bs1 has the distribution expected of a transposable element, different locations in different lines, and not that of a fixed gene locus. However, the common bands in the Zea mays lines and the recombinant inbred data imply that Bs1 is not highly mobile.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00224229

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Johns, M. A.

Springer

Journal of molecular evolution 30 (1990), S. 493-499

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ISSN: 1432-1432

Keywords: Maize ; Teosinte ; Ac ; Transposable elements

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Summary In light of previous data, which suggested thatAc-like sequences might have undergone a significant radiation in the recent past, I examined the copy number ofAc-like sequences in representatives of all theZea taxa, both maize and teosinte. The maize and teosinte samples contained approximately equal numbers ofAc-like sequences. FewAc-like sequences were in unmethylated regions of DNA. Unmethylated elements were distributed randomly among both maize and teosinte lines. The appearance in a line of a discrete band resulting from digestion with one methylation-sensitive restriction enzyme was correlated with the appearance of discrete bands with other methylation-sensitive bands. This suggests that individualAc-like elements are occasionally demethylated in many sites. No unmethylated element having restriction fragments of the lengths predicted from the publishedAc sequence was seen in the approximately 326 elements examined.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF02101104

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A Ds-mutation of the Adh1 gene in Zea mays L. (1984)

Döring, H. -P. ; Freeling, M. ; Hake, S. ; [et al.]

Springer

Molecular genetics and genomics 193 (1984), S. 199-204

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ISSN: 1617-4623

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Summary An unstable spontaneous mutation in the maize Adh1 gene, coding for alcohol dehydrogenase, was selected by allyl alcohol poisoning of wild type Adh1 pollen from a maize line carrying Ds at the Bz2 locus and one copy of Ac in an unknown position. The mutant has a null phenotype. No wild type pollen grains were detected in strains devoid of Ac, but in the presence of Ac, wild type pollen grains were detected with a frequency of between 10-4 and 10-3. In addition, events have been identified in the aleurone in which reversions of both bz2-m and the unstable adh1 mutation occurred in the same patch of tissue, presumably in response to an alteration of Ac. By these criteria, the Adh1 mutant is caused by Ds. DNA blotting experiments have shown the presence of a 1.3 kb insertion in the Adh1 gene. All or part of this Ds insertion is transcribed, and is detected as an insertion within the ADH1-mRNA. The longer mRNA hybridizes to an authentic Ds probe. This Ds element differs in size from other known Ds insertions.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00330667

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