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  • 1
    Electronic Resource
    Electronic Resource
    Genetic relationships in cultivars of hop, Humulus lupulus L., determined by RAPD analysis (1999)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 118 (1999), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Random amplified polymorphic DNA (RAPD) was used to evaluate the genetic variability and relationship of 65 hop cultivars from all the major hop-growing regions in the world. Twenty-eight selected random primers used in the RAPD reaction generated an average of 38.6%) polymorphic fragments, which was sufficient to produce 47 different RAPD profiles among the cultivars examined. The level of genetic variability was much higher than previously reported. Genetic similarity was estimated and UPGMA cluster analysis was performed using the RAPD data. Cluster analysis separated the cultivars into genetically related RAPD groups which were compared with pedigree data and grouping of the hop cultivars by essential oil type. The RAPD groups, strongly supported by pedigree data, gave more precise information on the level and distribution of genetic variability within hop cultivars than characterization by essential oils. Cultivars were divided into American and European groups, supporting the distinction between two geo-graphically distinct hop germplasms. Five genetically distinct groups revealed differences within the European germplasm, reflecting past hop breeding practices which have been adopted in different regions. The use of RAPD markers for hop germplasm characterization and genetic diversity study is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1439-0523.1999.118002175.x
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  • 2
    Electronic Resource
    Electronic Resource
    Screening for scab resistance by RAPD markers in cultivars of apple (Malus spp.) (1996)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 115 (1996), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Genetic markers are a much faster and more practical alternative to classical methods for the identification of genes for scab resistance present in different apple cultivars. In our study, 28 scab-resistant cultivars, four wild sources of the resistance genes and 10 susceptible cultivars were screened for the presence of the RAPD fragments OPM18/900, OPD20/600 and OPA15/900, which are reported to be linked to the Vf gene. All three marker fragments were successfully amplified with different protocols in Vf-resistant cultivars including ‘M. floribunda 821’. No marker fragments were amplified in susceptible cultivars, three out of four Va-resistant cultivars, three out of four Vm-resistant cultivars, two Vr-resistant cultivars, ‘Antonovka PI 172612’ and ‘M. pumila R 12740-7A’. All three markers were found in the cv. ‘Nova Easygro’, reported to possess the Vr gene, and the cv. ‘Reglindis’, reported to be Va-resistant. M. atrosanguinea of unknown origin showed the presence of OPD20/600 and OPA 15/900 marker bands. The cvs. ‘Nova Easygro’, ‘Reglindis’ and M. atrosanguinea are probably carriers of the VF gene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.1996.tb00963.x
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  • 3
    Electronic Resource
    Electronic Resource
    Second cycle gynogenesis in onion, Allium cepa L., and genetic analysis of the plants (1998)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 117 (1998), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Haploid induction in onion via gynogenesis provides inbred lines that can be used in production of hybrid cultivars. Despite several successful reports, the gynogenic induction rate is still relatively low, ranging mainly from 0.5 to six embryos per 100 cultured flowers. In the present study, gynogenesis was induced in selfed progeny of three genetically homozygous and stable fertile doubled haploid lines previously obtained through in vitro gynogenesis. The aims were to acquire information on whether the gynogenic responsiveness of a particular heterozygous cultivar is correlated with higher or lower gynogenic capacity in the second cycle of gynogenesis, to evaluate the non-genetic component of the variability, and to monitor by random amplified polymorphic DNA (RAPD) analysis the onset of eventual gametoclonal variation induced during the gynogenic processes. The results from the gynogenic induction of 9708 flowers showed significant differences among three lines, one of them being extremely responsive, showing a mean yield of 118.3 haploid embryos per 100 cultured flowers (118.3%). The high embryo yield of this line was determined by the line's particular genotype. However, the induction rate of gynogenic embryos obtained within 24 genetically uniform donor plants of this responsive line varied from 67 to 196% and this variation can be attributed to uncontrolled environmental conditions. A total of 157 plants sampled from two lines and obtained through a first (RI) and a second (SCG) cycle of gynogenesis, was analysed by RAPDs. The single band profiles were compared either between or within the RI and SCG plants of each line. A total number of 4600 RAPD fragments were scored and no variation was found in the highly responsive line, while in the less responsive one, minor changes were detected for the presence/absence of two RAPD bands. These results indicate high genetic stability of onion homozygous lines passed through two cycles of gynogenesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.1998.tb01939.x
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  • 4
    Electronic Resource
    Electronic Resource
    Gynogenic lines of onion (Allium cepa L.): evidence of their homozygosity (1995)
    Springer
    Theoretical and applied genetics 91 (1995), S. 598-602 
    Details
    ISSN: 1432-2242
    Keywords: Gynogenesis ; Isozyme ; RAPD ; Agronomic evaluation ; Gametoclonal variation ; Genetic homogeneity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Haploid induction via gynogenesis offers the possibility of using doubled haploid (DH) inbred lines in onion breeding. A first DH line that originated from the open-pollinated (OP) cultivar ‘Dorata di Parma’ was obtained after overcoming difficulties associated with the haploidy of the regenerants. Spontaneous chromosome doubling occurs seldom in onion. The first DH line obtained was cloned and selfed to produce sufficient seeds for genetic studies. The homozygosity of the DH gynogenic line was revealed on the basis of the low standard deviations of the bulb traits polar diameter, shape index and weight with respect to those of the S1 line or the OP cultivar. In the DH line, moreover, segregation of RAPD and alpha esterase markers was not noted. Out of four primers revealing polymorphism at 16 ge-netic loci in the OP cultivar ‘Dorata di Parma’, none produced polymorphism in the DH gynogenic line. The Est-1 locus, homozygous in 22 plants (Est-1 1/1 in 3 and Est-1 2/2 in 19) and heterozygous (Est-1 1/2) in 11 plants of the OP cultivar, always carried the same alleles in the DH line. We also tested genetic stability during micropropagation of a second halpoid line obtained via gynogenesis from var. ‘Senshyu Yellow’. Seventeen plants of this line were tested to detect changes occurring during the tissue culture process. Again no polymorphism was observed. The high genetic homogeneity observed in the two gynogenic lines of onion could be related to the absence of the callus phase during the gynogenic process.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223285
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  • 5
    Electronic Resource
    Electronic Resource
    A comparison of methods for identifying and surveying the presence of 1BL.1RS translocations in bread wheat (1991)
    Springer
    Euphytica 54 (1991), S. 45-53 
    Details
    ISSN: 1573-5060
    Keywords: Triucum aestivum ; wheat ; storage protein ; bread-making quality ; N-banding PA ; subtilisin inhibitor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Four methods for detecting 1BL. 1RS translocations in bread wheat have been compared winter wheat cultivars: N-banding of mitotic metaphase chromosomes, sodium lactate electrophoresis at pH 3.1, sodium dodecyl sulphate-polyacrylamide gel electrophorests under conditions, and a recently characterised protein, subtilisin inhibitor, separated by isoelectric N-banding was much the most labour intensive method, and, of the three electrophoretic recommend the use of subtilisin inhibitor, which is at least as easy to interpret as the other is often faster for screening purposes. The sources of the 1BL. 1RS translocation in this matenal Avrora. Kavkaz and Skorospelka 35, which have been extensively used as parents in breeding programmes. Out of 59 cultivars that include a line carrying the 1BL. 1RS pedigree. 23 of them did not carry the translocation; thus the effect on plant phenotype of insufficient to guarantee its selection during breeding.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00145630
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  • 6
    Electronic Resource
    Electronic Resource
    Gliadin allele composition of Yugoslav winter wheat cultivars (1991)
    Springer
    Euphytica 54 (1991), S. 285-295 
    Details
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; gliadins ; APAGE ; multiple allelism ; variation ; Yugoslavia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The complete gliadin allele composition of 57 Yugoslav common winter wheat cultivars was studied. Large differences were found in gliadin genotypes among cultivars bred at different Yugoslav breeding centres as well as between early and recent wheats bred in the Novi Sad breeding centre. Yugoslav wheats have limited variation in gliadin alleles, of which Gli-A1a, Gli-B1b, Gli-B1l, Gli-D1b, Gli-A2e, Gli-B2b, Gli-D2a are shown to be the most frequent. Examples of favoured alleles to new cultivars are described. 40% of the studied wheat cultivars have natural biotypes differing in gliadin allele composition. The frequency of cultivars with the biotypes differs greatly among breeding centres.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00023005
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  • 7
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    Molecular phylogeny of wild Hops, Humulus lupulus L. (2006)
    Springer Nature
    Details
    Publication Date: 2006-05-10
    Print ISSN: 0018-067X
    Electronic ISSN: 1365-2540
    Topics: Biology
    Published by Springer Nature
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  • 8
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    HELIOSEISMOLOGY OF PRE-EMERGING ACTIVE REGIONS. I. OVERVIEW, DATA, AND TARGET SELECTION CRITERIA (2012)
    Institute of Physics
    Details
    Publication Date: 2012-12-21
    Print ISSN: 0004-637X
    Electronic ISSN: 1538-4357
    Topics: Physics
    Published by Institute of Physics
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  • 9
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    Validating Forward Modeling and Inversions of Helioseismic Holography Measurements (2018)
    Institute of Physics
    Details
    Publication Date: 2018-08-08
    Print ISSN: 0004-637X
    Electronic ISSN: 1538-4357
    Topics: Physics
    Published by Institute of Physics
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  • 10
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    HELIOSEISMOLOGY OF PRE-EMERGING ACTIVE REGIONS. II. AVERAGE EMERGENCE PROPERTIES (2012)
    Institute of Physics
    Details
    Publication Date: 2012-12-21
    Print ISSN: 0004-637X
    Electronic ISSN: 1538-4357
    Topics: Physics
    Published by Institute of Physics
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