ISSN:
1572-9931
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract Using intraspecific hybrids, we have demonstrated the dominant nature of two phenotypic markers present in a mutant mouse 3T6 cell line, designated 3T6-V r B2. These are, resistance to virus infection (V r )and semiconstitutive synthesis of interferon (IF sc ).Hybrids were formed by polyethylene glycol-mediated fusion between 3T6-V r B2, or its parent 3T6, and 2TG0-13, a triply marked derivative of mouse 3T3 cells. When tested for the V r marker, 3T6-V r B2 ×2TG0-13 hybrid clones displayed a level of resistance to virus infection which was equal to or greater than that of 3T6-V r B2. Similarly, when tested for the IF sc marker, these hybrid clones were found to possess the capacity to confer an interferon-induced antiviral state in mouse L 929 cells upon cocultivation. By comparison, clones derived from 3T6 × 2TG0-13 fusions produced high levels of virus and failed to confer an interferon-induced antiviral state in L 929 cells.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01543158
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