ISSN:
1750-3841
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
A reliable enzyme assay was developed that could routinely be used to accurately reflect lactase activity. The assay was performed with a nonfat dry milk substrate at 37°C. This procedure was then utilized to evaluate the effects of potassium ions as a stabilizer and activator on four yeast lactase enzymes. Low levels of potassium ions (K+), incorporated as potassium chloride (KCl), increased enzyme units by 20% and 30% in two liquid lactases derived from Saccharomyces lactis. Levels as high as 9.4% KCl increased the activity of one liquid lactase as much as 160%. The K+ activated and stabilized a powdered form of lactase isolated from Kluyveromyces fragilis. The activity of the fourth lactase tested, also derived from S. Zactis, was slightly enhanced.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-2621.1984.tb12438.x
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