Publication Date:
2002-02-02
Description:
The Golgi apparatus is partitioned during mitosis in animal cells by a process of fragmentation, dispersal, and reassembly in each daughter cell. We fractionated the Golgi apparatus in vivo using the drug brefeldin A or a dominant-negative mutant of the Sar1p protein. After these treatments, Golgi enzymes moved back to the endoplasmic reticulum, leaving behind a matrix of Golgi structural proteins. Under these conditions, cells still entered and exited mitosis normally, and their Golgi matrix partitioned in a manner very similar to that of the complete organelle. Thus, the matrix may be the partitioning unit of the Golgi apparatus and may carry the Golgi enzyme-containing membranes into the daughter cells.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Seemann, Joachim -- Pypaert, Marc -- Taguchi, Tomohiko -- Malsam, Jorg -- Warren, Graham -- New York, N.Y. -- Science. 2002 Feb 1;295(5556):848-51.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Cell Biology, Ludwig Institute for Cancer Research, Yale University School of Medicine, 333 Cedar Street, Post Office Box 208002, New Haven, CT 06520-8002, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11823640" target="_blank"〉PubMed〈/a〉
Keywords:
Anaphase
;
Animals
;
Autoantigens
;
Brefeldin A/pharmacology
;
Cell Line
;
Endoplasmic Reticulum/enzymology
;
Golgi Apparatus/*metabolism/ultrastructure
;
HeLa Cells
;
Humans
;
Interphase
;
Intracellular Membranes/metabolism/ultrastructure
;
Mannosidases/metabolism
;
Membrane Proteins/metabolism
;
Metaphase
;
Microscopy, Electron
;
Microscopy, Fluorescence
;
*Mitosis
;
Monomeric GTP-Binding Proteins/pharmacology
;
N-Acetylglucosaminyltransferases/metabolism
;
Protein Disulfide-Isomerases/metabolism
;
Rats
;
*Saccharomyces cerevisiae Proteins
;
Telophase
;
Vesicular Transport Proteins
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
Permalink