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  • 1
    Publication Date: 2003-11-01
    Description: Functional analysis of a genome requires accurate gene structure information and a complete gene inventory. A dual experimental strategy was used to verify and correct the initial genome sequence annotation of the reference plant Arabidopsis. Sequencing full-length cDNAs and hybridizations using RNA populations from various tissues to a set of high-density oligonucleotide arrays spanning the entire genome allowed the accurate annotation of thousands of gene structures. We identified 5817 novel transcription units, including a substantial amount of antisense gene transcription, and 40 genes within the genetically defined centromeres. This approach resulted in completion of approximately 30% of the Arabidopsis ORFeome as a resource for global functional experimentation of the plant proteome.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Yamada, Kayoko -- Lim, Jun -- Dale, Joseph M -- Chen, Huaming -- Shinn, Paul -- Palm, Curtis J -- Southwick, Audrey M -- Wu, Hank C -- Kim, Christopher -- Nguyen, Michelle -- Pham, Paul -- Cheuk, Rosa -- Karlin-Newmann, George -- Liu, Shirley X -- Lam, Bao -- Sakano, Hitomi -- Wu, Troy -- Yu, Guixia -- Miranda, Molly -- Quach, Hong L -- Tripp, Matthew -- Chang, Charlie H -- Lee, Jeong M -- Toriumi, Mitsue -- Chan, Marie M H -- Tang, Carolyn C -- Onodera, Courtney S -- Deng, Justine M -- Akiyama, Kenji -- Ansari, Yasser -- Arakawa, Takahiro -- Banh, Jenny -- Banno, Fumika -- Bowser, Leah -- Brooks, Shelise -- Carninci, Piero -- Chao, Qimin -- Choy, Nathan -- Enju, Akiko -- Goldsmith, Andrew D -- Gurjal, Mani -- Hansen, Nancy F -- Hayashizaki, Yoshihide -- Johnson-Hopson, Chanda -- Hsuan, Vickie W -- Iida, Kei -- Karnes, Meagan -- Khan, Shehnaz -- Koesema, Eric -- Ishida, Junko -- Jiang, Paul X -- Jones, Ted -- Kawai, Jun -- Kamiya, Asako -- Meyers, Cristina -- Nakajima, Maiko -- Narusaka, Mari -- Seki, Motoaki -- Sakurai, Tetsuya -- Satou, Masakazu -- Tamse, Racquel -- Vaysberg, Maria -- Wallender, Erika K -- Wong, Cecilia -- Yamamura, Yuki -- Yuan, Shiaulou -- Shinozaki, Kazuo -- Davis, Ronald W -- Theologis, Athanasios -- Ecker, Joseph R -- New York, N.Y. -- Science. 2003 Oct 31;302(5646):842-6.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Plant Gene Expression Center, Albany, CA 94710, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/14593172" target="_blank"〉PubMed〈/a〉
    Keywords: Arabidopsis/*genetics ; Chromosome Mapping ; Chromosomes, Plant/genetics ; Cloning, Molecular ; Computational Biology ; DNA, Complementary/genetics ; DNA, Intergenic ; Expressed Sequence Tags ; Gene Expression Profiling ; Genes, Plant ; *Genome, Plant ; Genomics ; Nucleic Acid Hybridization ; Oligonucleotide Array Sequence Analysis ; Open Reading Frames ; RNA, Messenger/*genetics ; RNA, Plant/*genetics ; Reverse Transcriptase Polymerase Chain Reaction ; *Transcription, Genetic
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 2
    ISSN: 1520-6882
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Journal of radioanalytical and nuclear chemistry 110 (1987), S. 403-413 
    ISSN: 1588-2780
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract In using positrons as analytical tools the experimenter has two quite different options. The first and more obvious is to duplicate electron methods with positrons and see what differences (if any) result. The second is to exploit a unique characteristic of positrons, such as the formation and decay of the positronium atom, to study chemical composition and surface characteristics. Because positrons do not exist freely in our world, they must be obtained from radioactive sources or nuclear interactions. Source intensity has consequently been a limiting factor in experiments that attempt to duplicate electron applications. Some methods of producing and moderating positrons that have been developed here (and elsewhere) are described as well as results from studies using the sources. Surface measurements require less intense sources and yield useful data on materials such as xeolites, silica gels, graphite and alumina. Experimental apparatus, data and interpretation will be discussed.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 0142-2421
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Notes: When fast positrons from a radioactive source such as 58Co or 22Na are impinged on a suitably prepared moderator surface a measurable fraction (∼0.1%) is re-emitted as a monoenergetic beam. Several groups have efforts underway to implement this phenomenon as a practical monoenergetic positron gun to be used for surface studies. To address the question that arises about the effects of the window of the capsule that contains the fast positron source, moderated positron intensities have been measured for windows constructed of several different materials of varying thicknesses. A semilog plot of the data approximates a universal curve, with all points on the same line regardless of material. A 58Co source can be masked by windows as thick as 22 µm Al or 11 µm Ti without decresasing the moderated positron intensity by more than 35%. Since 22Na has a similar fast β+ spectrum we have the implication that window effects for sources of this material will be of the same degree.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 0142-2421
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Notes: There is reason to believe that slow positron spectroscopy will have value for surface analyses, but in order to develop practical methods it is necessary to devise monoenergetic sources of positrons that output usable intensities. This paper describes how this can be achieved by preparing certain metal surfaces to act as moderators for fast positrons from radioactive sources. After entering the moderator, the positrons are thermalized and a certain fraction are re-emitted back into the vacuum. They can then be extracted by an electrostatic field and accelerated to any desired energy. Yields of low energy positrons were found to be generally large for certain metals having negative positron work functions (theoretically calculated). It is also necessary to minimize bulk defects, which act as positron traps. Tungsten and molybdenum are metals having negative work functions which can be easily prepared as effective moderators by simply heating in vacuum. This effects a cleaning by volatilizing oxides and anneals defects. The inertness of their surfaces towards oxidation allows them to maintain the negative work function effect which might otherwise be reduced by exposure to air and poor vacuum. These results have been utilized by the authors to design a monoenergetic positron gun to measure discrete energy losses in reflection spectra and threshold absorption effects. A Brandeis University group has used a tungsten moderator gun to make the first experimental measurements of low energy positron diffraction spectra.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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  • 6
    Publication Date: 2004-10-04
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
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  • 7
    Publication Date: 2014-12-27
    Description: Giant molecular clouds (GMCs) are observed to be turbulent, but theory shows that without a driving mechanism turbulence should quickly decay. The question arises by which mechanisms turbulence is driven or sustained. It has been shown that photoionizing feedback from massive stars has an impact on the surrounding GMC and can for example create vast H ii bubbles. We therefore address the question of whether turbulence is a consequence of this effect of feedback on the cloud. To investigate this, we analyse the velocity field of simulations of high-mass star-forming regions by studying velocity structure functions and power spectra. We find that clouds whose morphology is strongly affected by photoionizing feedback also show evidence of driving of turbulence by preserving or recovering a Kolmogorov-type velocity field. On the contrary, control run simulations without photoionizing feedback have a velocity distribution that bears the signature of gravitational collapse and of the dissipation of energy, where the initial Kolmogorov-type structure function is erased.
    Print ISSN: 0035-8711
    Electronic ISSN: 1365-2966
    Topics: Physics
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  • 8
    Publication Date: 2016-04-14
    Description: Acropora cervicornis, a threatened, keystone reef-building coral has undergone severe declines (〉90 %) throughout the Caribbean. These declines could reduce genetic variation and thus ...
    Electronic ISSN: 1471-2164
    Topics: Biology
    Published by BioMed Central
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  • 9
    Publication Date: 2016-09-02
    Description: We present an analysis of the effect of feedback from O- and B-type stars with data from the integral field spectrograph Multi Unit Spectroscopic Explorer (MUSE) mounted on the Very Large Telescope of pillar-like structures in the Carina Nebular Complex, one of the most massive star-forming regions in the Galaxy. For the observed pillars, we compute gas electron densities and temperatures maps, produce integrated line and velocity maps of the ionized gas, study the ionization fronts at the pillar tips, analyse the properties of the single regions, and detect two ionized jets originating from two distinct pillar tips. For each pillar tip, we determine the incident ionizing photon flux Q 0, pil originating from the nearby massive O- and B-type stars and compute the mass-loss rate $\dot{M}$ of the pillar tips due to photoevaporation caused by the incident ionizing radiation. We combine the results of the Carina data set with archival MUSE data of a pillar in NGC 3603 and with previously published MUSE data of the Pillars of Creation in M16, and with a total of 10 analysed pillars, find tight correlations between the ionizing photon flux and the electron density, the electron density and the distance from the ionizing sources, and the ionizing photon flux and the mass-loss rate. The combined MUSE data sets of pillars in regions with different physical conditions and stellar content therefore yield an empirical quantification of the feedback effects of ionizing radiation. In agreement with models, we find that $\dot{M}\propto Q_\mathrm{0,pil}^{1/2}$ .
    Print ISSN: 0035-8711
    Electronic ISSN: 1365-2966
    Topics: Physics
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  • 10
    Publication Date: 2014-12-29
    Description: Giant molecular clouds (GMCs) are observed to be turbulent, but theory shows that without a driving mechanism turbulence should quickly decay. The question arises by which mechanisms turbulence is driven or sustained. It has been shown that photoionizing feedback from massive stars has an impact on the surrounding GMC and can for example create vast H ii bubbles. We therefore address the question of whether turbulence is a consequence of this effect of feedback on the cloud. To investigate this, we analyse the velocity field of simulations of high-mass star-forming regions by studying velocity structure functions and power spectra. We find that clouds whose morphology is strongly affected by photoionizing feedback also show evidence of driving of turbulence by preserving or recovering a Kolmogorov-type velocity field. On the contrary, control run simulations without photoionizing feedback have a velocity distribution that bears the signature of gravitational collapse and of the dissipation of energy, where the initial Kolmogorov-type structure function is erased.
    Print ISSN: 0035-8711
    Electronic ISSN: 1365-2966
    Topics: Physics
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