Publication Date:
1991-10-18
Description:
The guanosine triphosphate (GTP)-binding proteins include signal-transducing heterotrimeric G proteins (for example, Gs, Gi), smaller GTP-binding proteins that function in protein sorting, and the oncogenic protein p21ras. The T cell receptor complexes CD4-p56lck and CD8-p56lck were found to include a 32- to 33-kilodalton phosphoprotein (p32) that was recognized by an antiserum to a consensus GTP-binding region in G proteins. Immunoprecipitated CD4 and CD8 complexes bound GTP and hydrolyzed it to guanosine diphosphate (GDP). The p32 protein was covalently linked to [alpha-32P]GTP by ultraviolet photoaffinity labeling. These results demonstrate an interaction between T cell receptor complexes and an intracellular GTP-binding protein.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Telfer, J C -- Rudd, C E -- New York, N.Y. -- Science. 1991 Oct 18;254(5030):439-41.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Division of Tumor Immunology, Dana-Farber Cancer Institute, Boston, MA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/1925604" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Sequence
;
Antigens, CD4/*metabolism
;
Antigens, CD8/*metabolism
;
Cell Line
;
GTP-Binding Proteins/*metabolism
;
Humans
;
Lymphocyte Specific Protein Tyrosine Kinase p56(lck)
;
Molecular Sequence Data
;
Phosphoproteins/metabolism
;
Precipitin Tests
;
Protein Binding
;
Protein-Tyrosine Kinases/*metabolism
;
Receptors, Antigen, T-Cell/*metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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