ISSN:
1573-5028
Keywords:
DNA synthesis
;
germination
;
single-strand breaks
;
nucleoid sedimentation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract DNA synthesis was studied during germination by following the rate of incorporation of radioactive thymidine into high molecular weight DNA. A peak of DNA synthesis was observed between the 8th and the 12th hour, i.e. before the beginning of the semi-conservative replication of genomic DNA, accompanied by an increase in the DNA content of the embryo. By the use of nucleoid sedimentation and nick-translation it was shown that, during the first hours of germination, extensive repair occurs of the DNA single-strand breaks present in the dry embryo. As a result, the DNA of the 16-h-germinated embryo acquires the conformation typical of that of the root meristemic cells active in transcription and replication. In addition we have shown that cytoplasmic organelle (most probably mitochondrial) DNA synthesis is very active during the prereplicative state which confirms earlier microscopic data on mitochondrial biogenesis during early germination.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00016151
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