ISSN:
0886-1544
Keywords:
sea urchin
;
centrosome
;
immunofluorescence microscopy
;
barrel-shaped spindle
;
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Medicine
Notes:
T-1 induces modifications in the shape of the centrosome at division in fertilized eggs of the North American sea urchin, Lytechinus pictus. Phase contrast microscopy observations of mitotic apparatus isolated from T-1treated (1.7-8.5μM) eggs at first division shows that the centrosomes already begin to spread or to separate by prophase and that the mitotic spindle is barrel-shaped. When eggs are fertilized with sperm that have been pretreated with T-1, the centrosomes become flattened; the spindles are of normal length. Immunofluorescence microscopy using an anti-centrosomal monoclonal antibody reveals that T-l modifies the structure of the centrosome so that barrel-shaped spindles with broad centrosomes are observed at metaphase, rather than the expected focused poles and fusiform spindle. Higher concentrations of T-l induce fragmentation of centrosomes, causing abnormal accumulation of microtubules in polar regions. These results indicate that T-l directly alters centrosomal configuration from a compact structure to a flattened or a spread structure. T-l can be classified as a new category of mitotic drugs that may prove valuable in dissecting the molecular nature of centrosomes.
Additional Material:
4 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/cm.970160208
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