ISSN:
1574-695X
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
,
Medicine
Notes:
We have recently demonstrated that the cell wall β-glucan of Candida albicans could be solubilized by sodium hypochlorite, followed by dimethylsulfoxide-extraction (NaClO-DMSO method). In this study, applying this method to Aspergillus spp., we prepared mycelial cell wall β-glucan and examined its physical properties and immunotoxicological activity. The acetone-dried mycelia of Aspergillus spp. were oxidized by the NaClO-DMSO method. An analysis of 13C NMR spectra revealed the preparations to be composed of α-(1→3) and β-(1→3)-d-glucan. Also, the proportion of α-(1→3) and β-(1→3)-d-glucan varied. Futhermore, a solubilized Aspergillusβ-glucan (ASBG) was prepared from OX-Asp by urea-autocalve treatment. ASBG showed limulus activity similar to Candida solubilized β-glucan (CSBG), and there was little difference in the activity of ASBG between various Aspergillus spp. ASBG affected the production of IL-8 by human peripheral blood mononuclear cells (PBMC). ASBG should be useful for analyzing the clinical role of β-glucan.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1016/j.femsim.2004.04.004
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