ISSN:
1432-0614
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Summary Plant cell suspensions of Catharanthus roseus efficiently converted exogenously supplied hydroquinone (HQ) into arbutin. Arbutin productivity of the cells was strongly influenced by the growth stage of the cultivated cells and by the manner of the addition of HQ. We have developed two methods: (i) cultivating suitable cells for producing arbutin at high density; (ii) efficiently adding toxic HQ to the cells. The yield of arbutin could be increased up to 9.2 g/l (45% of cell dry weight), which is the highest yield in the field of plant biotechnology. Repeated examinations and scaling up to a 20-l jar fermentor suggested that C. roseus cells stably produce arbutin in large amounts under the established conditions.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00208148
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