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1

Digitale Medien

Protein kinase C (α, β, γ) in Pacinian corpuscle (1992)

Kawakita, Naoto ; Mizoguchi, Akira ; Masutani, Motomaru ; [weitere]

Springer

Histochemistry and cell biology 98 (1992), S. 381-387

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ISSN: 1432-119X

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Medizin

Notizen: Abstract Immunocytochemical demonstration of protein kinase C (PKC) subspecies (α, β, γ) was carried out in Pacinian corpuscles of rat hind feet using monoclonal or polyclonal antibodies against each of these subspecies. The inner core cells and lamellae and the Schwann cell cytoplasm of the nerve fiber innervating the corpuscle were strongly positive for PKC α-immunoreactivity (IR). In contrast, the axon terminal and the outer core did not display any positive α-IR. Very weak PKC β-IR was detected in the ultraterminal region of the axon terminal, while the trunk region showed no immunoreactivity. Very faint PKC β-IR was found also in the lamellar cells located at the periphery of the inner core and the endoneurial fibroblasts in the intermediate layer. PKC γ-IR was not detected in any part of the corpuscle. The strong PKC α-IR in the inner core and the presence of absence of PKC α-, β-, and γ-IR in the axon terminal are discussed from the point of view of the functional aspects of each part.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00271074

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2

Digitale Medien

Localization of synapsin I in normal fibers and regenerating axonal sprouts of the rat sciatic nerve (1996)

Akagi, Shuichiro ; Mizoguchi, Akira ; Sobue, Kenji ; [weitere]

Springer

Histochemistry and cell biology 105 (1996), S. 365-373

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ISSN: 1432-119X

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Medizin

Notizen: Abstract The localization of synapsin I, a synaptic vesicle-associated protein, was investigated immunocytochemically in normal nerve fibers and regenerating axonal sprouts following crush-injuries to the rat sciatic nerve. In normal myelinated axons, weak synapsin I immunoreactivity was found in the axoplasmic/smooth endoplasmic domains, but not in the cytoskeletal domains comprising neurofilaments and microtubules. In non-myelinated axons without dense cytoskeletal structures, moderate immunoreactivity was distributed diffusely throughout the axoplasm. In the crush-injured nerves, intense synapsin I immunoreactivity was demonstrated by light microscopy in early regenerating sprouts emerging from nodes of Ranvier. These nodal sprouts subsequently elongated as regenerating axons through the space between the basal lamina and the myelin sheath (or Schwann cell plasma membrane). Intense synapsin I immunoreactivity was also found in the growth cones of such long regenerating axons. Electron microscopy revealed that synapsin I immunoreactivity was associated mainly with vesicular organelles in the nodal sprouts and growth cones of regenerating axons. Long regenerating axons exhibited no synapsin I immunoreactivity in the shaft, which contained an abundance of neurofilaments. However, vesicle accumulations remaining in the periphery of the shaft still exhibited intense synapsin I immunoreactivity. Thus, it can be concluded that synapsin I is localized at especially high density in the domains comprising vesicular organelles, which are characteristic of early nodal sprouts, as well as in growth cones of regenerating axons. These findings, together with the proposed functions of synapsin I investigated in other studies, suggest that synapsin I may play important roles in vesicular dynamics including the translocation of vesicles to the plasma membrane in sprouts and growth cones of regenerating axons.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF01463657

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3

Digitale Medien

Developmental changes in the subcellular localization of R-cadherin in chick retinal pigment epithelium (1997)

Liu, X. ; Mizoguchi, Akira ; Takeichi, Masatoshi ; [weitere]

Springer

Histochemistry and cell biology 108 (1997), S. 35-43

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ISSN: 1432-119X

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Medizin

Notizen: Abstract It has been reported that in the chick embryonic retina, N-cadherin first appears at the very early stages and is subsequently substituted by R-cadherin at the middle to late stages of development. To examine the role of R-cadherin in the morphogenesis of chick retinal pigment epithelium (RPE), the distribution of this adhesion molecule was studied by immunofluorescence cytochemistry and immunoelectron microscopy from embryonic day (E) 6 to hatching. R-cadherin immunoreactivity was detected at E6, and was strongest at E12–13. During these stages, R-cadherin was expressed uniformly on the lateral plasma membranes of RPE cells in contact with each other. Thereafter, R-cadherin immunoreactivity was markedly decreased, with intense immunoreactivity restricted to zonulae adherentes in latero-apical regions at E16. R-cadherin immunoreactivity was no longer detectable in the newly hatched chick RPE, even though morphologically well developed zonulae adherentes were present in latero-apical regions. No immunoreactivity was detected on the apical side facing the neural retina or on the basal side facing the basal lamina at any stage of development. These findings indicate that R-cadherin plays an important role as a major cadherin subtype in the morphogenesis of chick embryo RPE, and is involved initially in non-specific cell-cell adhesions, and subsequently in the formation and maintenance of developing zonulae adherentes.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/s004180050144

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Digitale Medien

The development of melanosomes in the pigment epithelium of the chick embryo (1972)

Ide, Chizuka

Springer

Cell & tissue research 131 (1972), S. 171-186

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ISSN: 1432-0878

Schlagwort(e): Pigment epithelium ; Premelanosome ; Golgi complex ; Cytochemistry ; Tyrosinase

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Medizin

Notizen: Summary The origin of the melanosome in the pigment epithelium of the chick embryo was studied by electron microscopy and cytochemistry of tyrosinase. The melanosome appears first at stage 16 in the dorso-caudal region of the optic cup and the first appearance of the tyrosinase activity can also be detected at the same stage in Golgi sac. At the early stages, premelanosomes and amorphous, electron dense granules which are considered to be the developing premelanosomes appear as a group at the basal region of the outer layer cell. The membrane of these granules is connected with that of ER. Attention should be paid to the fact that there are tyrosinase-negative premelanosomes, even when Golgi sac and Golgi vesicles are tyrosinase-positive. According to these facts it can be said that the site of origin of premelanosomes are not Golgi vesicles, but the smooth-surfaced ER connected with the rough-surfaced ER, and that the tyrosinase is transported to premelanosomes by tyrosinase-containing vesicles which originate from matured Golgi sac.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00306925

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5

Digitale Medien

Three-dimensional organization of the collagen fibrils in the rat sciatic nerve as revealed by transmission- and scanning electron microscopy (1990)

Ushiki, Tatsuo ; Ide, Chizuka

Springer

Cell & tissue research 260 (1990), S. 175-184

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ISSN: 1432-0878

Schlagwort(e): Collagen fibrils ; Sciatic nerve ; Epineurium ; Perineurium ; Endoneurium ; Scanning electron microscopy ; Rat (Wistar)

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Medizin

Notizen: Summary The organization of collagen fibrils in the rat sciatic nerve was studied by scanning electron microscopy after digestion of cellular elements by sodium hydroxide treatment, and by conventional transmission electron microscopy. The epineurium consisted mainly of thick bundles of collagen fibrils measuring about 10–20 μm in width; they were wavy and ran slightly obliquely to the nerve axis. Between these collagen bundles, a very coarse meshwork of randomly oriented collagen fibrils was present. In the perineurium, collagen fibrils occupied the interspaces between the concentrically arranged perineurial cells; in each interspace, they formed a sheet of characteristic lacework elaborately interwoven by thin (about 3 μm or less in width) bundles of collagen fibrils. In the subperineurial region, there was a distinct sheet of densely woven collagen fibrils between the perineurium and underlying endoneurial fibroblasts. In the endoneurium, collagen fibrils surrounded individual nerve fibers in two layers as scaffolds: the inner layer was made up of a delicate meshwork of very fine collagen fibrils, and the outer one consisted of longitudinally oriented bundles of about 1–3 μm in width. The collagen fibril arrangement described above may protect the nerve fibers against external forces.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00297503

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6

Digitale Medien

A re-evaluation of the cytology of cat Pacinian corpuscles (1988)

Munger, Bryce L. ; Yoshida, Yasuo ; Hayashi, Shuichiro ; [weitere]

Springer

Cell & tissue research 253 (1988), S. 83-93

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ISSN: 1432-0878

Schlagwort(e): Pacinian corpuscle ; Axonal spines ; Inner core lamella ; Inner core cleft ; Elastica ; Cat

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Medizin

Notizen: Summary The ultrastructure of cat mesenteric Pacinian corpuscles in cross and longitudinal sections has been examined. The terminal ends of lamellar cells of the inner core have been identified in longitudinal sections through the proximal portion of the inner core. These terminal bulbous expansions contain characteristic concentric membranes of rough endoplasmic reticulum and in some cases masses of oval membranous inclusions. The central axon as seen in cross section is oval in profile, having X-(short) and Y-(long) axes, and each axonal face is characterized by specializations of the axolemma. At the X-axis, the inner lamellae of the inner core tightly abut a smooth axolemma, with no intervening connective tissue matrix, in a manner reminiscent of a neuroepithelium. The axolemma of the Y-axis has numerous axonal spines (microspikes) that project into the cleft in the inner core. The extent of the axolemma having axonal spines can only be appreciated in longitudinal sections. The clefts contain a specialized connective tissue with elastic and collagen fibrils. The connective tissue compartment of fibers and matrix separating individual inner core lamellae is unique, in that it contains extremely thin collagen fibrils measuring approximately 15 nm in diameter. The diameter of collagen fibrils increases as the cleft is approached. Here the fibrils resemble typical endoneural collagen.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00221743

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7

Digitale Medien

A re-evaluation of the cytology of cat Pacinian corpuscles (1988)

Ide, Chizuka ; Yoshida, Yasuo ; Hayashi, Shuichiro ; [weitere]

Springer

Cell & tissue research 253 (1988), S. 95-103

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ISSN: 1432-0878

Schlagwort(e): Pacinian corpuscle ; Axonal spines ; Axonal extreme tip ; Inner core lamella ; Basal lamina-like material ; Cat

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Medizin

Notizen: Summary The present report is the second of two studies re-evaluating the cytological characteristics of Pacinian corpuscles. The extreme tip of the axon of a Pacinian corpuscle has been identified and is quite different from the previously described ultraterminal region. The latter is the site where the inner core lamellae begin to terminate and is characterized by a smooth axolemma. The extreme tip lacks inner core lamellae directly abutting the axolemma and is instead characterized by the presence of many axonal spines projecting into a matrix of basal lamina-like material. The extreme tip of the axon thus resembles the organization of the axolemma facing the clefts of the inner core. The axonal spines at the cleft and extreme tip are proposed as a site of restricted current flow due to the tight apposition of inner core lamellae to the axolemma of X-axis. The hemiinner cores thus could restrict current flow to the cleft. These anatomical specializations could represent both a source and a sink for K+ ions during mechano-electric transduction and account in part for the exquisite sensitivity of Pacinian corpuscles to complex pressure waves.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00221744

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