ISSN:
1476-4687
Source:
Nature Archives 1869 - 2009
Topics:
Biology
,
Chemistry and Pharmacology
,
Medicine
,
Natural Sciences in General
,
Physics
Notes:
[Auszug] To purify PHF, 30-40 g of frozen AD cortex rich in neurofibrillary tangles were finely chopped, incubated at 25 C for 2h in 50 mM Tris pH7.6, 0.1M b-mercaptoethanol (b-ME), 2% SDS (SDS/b-ME buffer), Dounce-homogenized and heated to 100 C for 5 min. The homogenate was seived once through nylon ...
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1038/304727a0
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