Publication Date:
2014-08-01
Description:
The evolutionary relationships of extinct species are ascertained primarily through the analysis of morphological characters. Character inter-dependencies can have a substantial effect on evolutionary interpretations, but the developmental underpinnings of character inter-dependence remain obscure because experiments frequently do not provide detailed resolution of morphological characters. Here we show experimentally and computationally how gradual modification of development differentially affects characters in the mouse dentition. We found that intermediate phenotypes could be produced by gradually adding ectodysplasin A (EDA) protein in culture to tooth explants carrying a null mutation in the tooth-patterning gene Eda. By identifying development-based character inter-dependencies, we show how to predict morphological patterns of teeth among mammalian species. Finally, in vivo inhibition of sonic hedgehog signalling in Eda null teeth enabled us to reproduce characters deep in the rodent ancestry. Taken together, evolutionarily informative transitions can be experimentally reproduced, thereby providing development-based expectations for character-state transitions used in evolutionary studies.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4252015/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4252015/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Harjunmaa, Enni -- Seidel, Kerstin -- Hakkinen, Teemu -- Renvoise, Elodie -- Corfe, Ian J -- Kallonen, Aki -- Zhang, Zhao-Qun -- Evans, Alistair R -- Mikkola, Marja L -- Salazar-Ciudad, Isaac -- Klein, Ophir D -- Jernvall, Jukka -- DP2 OD007191/OD/NIH HHS/ -- DP2-OD007191/OD/NIH HHS/ -- K99 DE024214/DE/NIDCR NIH HHS/ -- R01 DE021420/DE/NIDCR NIH HHS/ -- R01-DE021420/DE/NIDCR NIH HHS/ -- England -- Nature. 2014 Aug 7;512(7512):44-8. doi: 10.1038/nature13613. Epub 2014 Jul 30.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Developmental Biology Program, Institute of Biotechnology, University of Helsinki, P.O. Box 56, FIN-00014 Helsinki, Finland. ; 1] Program in Craniofacial and Mesenchymal Biology, University of California, San Francisco, San Francisco, California 94114, USA [2] Department of Orofacial Sciences, University of California, San Francisco, San Francisco, California 94114, USA. ; Division of Materials Physics, Department of Physics, University of Helsinki, P.O. Box 64, FIN-00014 Helsinki, Finland. ; Key Laboratory of Evolutionary Systematics of Vertebrates, Institute of Vertebrate Paleontology and Paleoanthropology, Chinese Academy of Sciences, Beijing 100044, China. ; 1] School of Biological Sciences, Monash University, Victoria 3800, Australia [2] Geosciences, Museum Victoria, GPO Box 666, Melbourne, Victoria 3001, Australia. ; 1] Developmental Biology Program, Institute of Biotechnology, University of Helsinki, P.O. Box 56, FIN-00014 Helsinki, Finland [2] Genomics, Bioinformatics and Evolution Group. Department de Genetica i Microbiologia, Universitat Autonoma de Barcelona, Cerdanyola del Valles 08193, Spain. ; 1] Program in Craniofacial and Mesenchymal Biology, University of California, San Francisco, San Francisco, California 94114, USA [2] Department of Orofacial Sciences, University of California, San Francisco, San Francisco, California 94114, USA [3] Department of Pediatrics, University of California, San Francisco, San Francisco, California 94114, USA [4] Institute for Human Genetics, University of California, San Francisco, San Francisco, California 94114, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/25079326" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
*Biological Evolution
;
Computer Simulation
;
Ectodysplasins/deficiency/genetics/pharmacology
;
Female
;
*Fossils
;
Gene Deletion
;
Hedgehog Proteins/antagonists & inhibitors/genetics
;
In Vitro Techniques
;
Male
;
Mice
;
Molar/anatomy & histology/drug effects/growth & development
;
Phenotype
;
Signal Transduction/drug effects
;
Tooth/*anatomy & histology/drug effects/*growth & development
Print ISSN:
0028-0836
Electronic ISSN:
1476-4687
Topics:
Biology
,
Chemistry and Pharmacology
,
Medicine
,
Natural Sciences in General
,
Physics
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