Publication Date:
2002-07-06
Description:
The transcription factor Pax5 is essential for initiating B cell lineage commitment, but its role in maintaining commitment is unknown. Using conditional Pax5 inactivation in committed pro-B cells, we demonstrate that Pax5 is required not only to initiate its B lymphoid transcription program, but also to maintain it in early B cell development. As a consequence of Pax5 inactivation, previously committed pro-B cells regained the capacity to differentiate into macrophages in vitro and to reconstitute T cell development in vivo in RAG2-/- mice. Hence, Pax5 expression is continuously required to maintain B cell lineage commitment, because its loss converts committed pro-B cells into hematopoietic progenitors with multilineage potential.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Mikkola, Ingvild -- Heavey, Barry -- Horcher, Markus -- Busslinger, Meinrad -- New York, N.Y. -- Science. 2002 Jul 5;297(5578):110-3.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Research Institute of Molecular Pathology, Vienna Biocenter, Dr. Bohr-Gasse 7, A-1030 Vienna, Austria.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/12098702" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Antigens, CD19/genetics/metabolism
;
B-Cell-Specific Activator Protein
;
B-Lymphocytes/cytology/*physiology
;
Cell Differentiation
;
Cell Lineage
;
Cells, Cultured
;
DNA-Binding Proteins/*genetics/*metabolism
;
Gene Expression Profiling
;
Gene Expression Regulation
;
Gene Silencing
;
Hematopoietic Stem Cells/cytology/*physiology
;
Macrophages/cytology/physiology
;
Mice
;
Mice, Inbred C57BL
;
Mice, Transgenic
;
T-Lymphocytes/cytology/physiology
;
Tamoxifen/*analogs & derivatives/pharmacology
;
Transcription Factors/*genetics/*metabolism
;
Transcription, Genetic
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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