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  • 1
    Electronic Resource
    Electronic Resource
    Kinetics of Nitrobacter agilis at extreme substrate, product and salt concentrations (1993)
    Springer
    Applied microbiology and biotechnology 40 (1993), S. 442-448 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Measurement and description of the effects of extreme conditions on biological nitrite oxidation was the aim of this study, using Nitrobacter agilis (ATCC 14123) as a model nitrifying bacterium. The initial O2 consumption rate of a concentrated cell suspension was used as a rapid assay to measure the effects. Several relationships, based on Michaelis-Menten kinetics, were derived. These relationships describe the behaviour of N. agilis with respect to substrate inhibition, product inhibition and various salt concentrations up to 500 mmol · dm −3 with the pH ranging from 6.5 to 8.5. Substrate and product inhibition were pH-dependent and the substrate inhibition could be related to the undissociated nitrite. In contrast with previous reports on nitrite-oxidizing microorganisms, we did not observe severe inhibition by NH inf4 sup+ .
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00170408
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  • 2
    Electronic Resource
    Electronic Resource
    Kinetics of Nitrosomonas europaea at extreme substrate, product and salt concentrations (1992)
    Springer
    Applied microbiology and biotechnology 37 (1992), S. 802-807 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Nitrification of ammonia in concentrated waste streams is gaining a lot of attention nowadays. Nitrosomonas europaea is the predominant ammonia-oxidizing species in these environments. Prediction of the behaviour of a pure culture of N. europaea (ATCC 19718) under conditions prevailing in concentrated waste streams was the aim of this study. The initial oxygen consumption rate of a concentrated cell suspension was used as a rapid assay to measure the effects on N. europaea under various conditions. Several relationships, based on Michaelis-Menten kinetics, were derived. They describe the behaviour of N. europaea at substrate (NH4 +), product (NO2 − and K+, Na+, SO inf4 sup2− , NO3 −, Cl−) concentrations up to 500 mol/m3 and pHs ranging from 6.5 to 8.5. High concentrations of ions inhibited N. europaea but specific substrate inhibition was not observed. Product inhibition was strongly pH-dependent and severe inhibition was found at pH 6.5.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00174849
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  • 3
    Electronic Resource
    Electronic Resource
    Effects of diffusion limitation on immobilized nitrifying microorganisms at low temperatures (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 45 (1995), S. 1-9 
    Details
    ISSN: 0006-3592
    Keywords: nitrification ; immobilized cells ; activation energy ; diffusion limitation ; temperature ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Activation energies of suspended and immobilized nitrifying bacteria were determined and compared to determine if diffusion limitation results in decreased sensitivity for temperature. The activation energy for the respiration activity of suspended Nitrosomonas europaea and Nitrobacter agilis was found to be 86.4 and 58.4 kJ mol-1, respectively. The activation energy for oxygen diffusion in the support material, κ-carrageenan, determined from the effect of temperature on the effective diffusion coefficient (D), was 17.2 kJ mol-1. Consequently, the apparent actvation energy of diffusion limited cells should be lower. It was indeed shown that due to the effect of diffusion limitation and to temperature effects on the Monod constant Ks, the immobilized-cell activity was less sensitive to temperature. The apparent activation energy for immobilized Ns. europaea was between 28.6 and 94.2 kJ mol-1 and for immobilized Nb. agilis between 1.4 and 72.9 kJ mol-1, depending on the oxygen concentration and temperature. © 1995 John Wiley & Sons, Inc.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260450102
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