Publication Date:
2009-11-20
Description:
Abstract 2269 Poster Board II-246 Background. Acute graft-versus-host disease (GVHD) has remained a significant cause of nonrelapse mortality after allogeneic hematopoietic cell transplantation (HCT) with nonmyeloablative conditioning. The role of tumor necrosis factor-alpha (TNF-α) in the biology of acute GVHD following nonmyeloablative conditioning has not been studied thus far. Here, we measured TNF receptor 1 (TNFR1) as a surrogate marker for TNF-α in 106 patients before the start of the conditioning regimen (baseline) and 7 days after allogeneic HCT following nonmyeloablative conditioning. Patients and Methods. The nonmyeloablative regimen consisted of 2 Gy total body irradiation (TBI) alone (n=15), 2 Gy TBI plus fludarabine 90 mg/m2 (n=18). Postgrafting immunosuppression combined mycophenolate mofetil (MMF) with a calcineurin inhibitor for all patients. Blood samples were prospectively collected before the start of the conditioning regimen, then on days 7, 14, 21, 28, 35, 42, 49, 56, 63, 70, 77, 84, 91, and 98 after HCT, and then generally once every 2 weeks up to day 180. The serum component of each blood sample was separated and frozen for later analysis on the day of sample acquisition. TNFR1 serum concentration was retrospectively assessed using a cytokine enzyme-linked immunoabsorbent assay (R&D, Minneapolis, MN) according to the manufacturer's protocol. Results. TNFR1 levels increased significantly from baseline to day 7 after nonmyeloablative HCT (P
Print ISSN:
0006-4971
Electronic ISSN:
1528-0020
Topics:
Biology
,
Medicine
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